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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | CFPAC-1 is a human pancreatic adenocarcinoma cell line derived from liver metastases in a 26-year-old Caucasian male patient with cystic fibrosis. It is the first pancreatic cancer cell line established from a patient with cystic fibrosis. The significance of this cell line lies in its expression of the cystic fibrosis transmembrane transport regulator (CFTR) and its presence of the common ΔF508 mutation. CFPAC-1 cells exhibit epithelial cell morphology and retain characteristics of ductal cell origin, including the ability to form polarized monolayers and secrete specific mucins. It is a valuable model for studying the link between CFTR dysfunction and pancreatic malignancies, as well as pancreatic cancer progression and ion transport. |
| Tissue | Pancreas; derived from metastatic site (Liver) |
| Disease | Adenocarcinoma; Pancreatic Cancer; Cystic Fibrosis |
| Morphology | Epithelial |
| Gender | Male |
| Age | 26 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Research on CFTR protein function and ΔF508 mutation 2. Research on pancreatic cancer metastasis and invasion 3. Drug screening for cystic fibrosis and pancreatic adenocarcinoma 4. Research on ion transport and epithelial electrolyte secretion 5. Research on mucin production in cancer cells |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Hypotriploid; Modal number = 54–59 |
| Tumorigenic | Yes, in nude mice |
| Genetic Profile | CFTR mutation; KRAS mutation; TP53 mutation |
| Expression Markers | Cytokeratin positive; Mucin (MUC1) expression |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with Ca2+/Mg2+-free PBS or 0.25% trypsin-EDTA solution to remove residual serum. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution and observe under an inverted microscope until the cell layer disperses (usually 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently aspirate the cells using a pipette. 5. Aliquot the cell suspension into new culture containers. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Medium | Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% Fetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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|---|---|---|
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