NCI-H661 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	NCI-H661 is an epithelioid cell line isolated from the lung of a 43-year-old Caucasian male with lung cancer. NCI-H661 cells lack ultrastructural and biochemical evidence of mucin production and squamous differentiation. NCI-H661 cells express easily detectable p53 mRNA at levels significantly higher than those of normal lung cells, while lacking gross structural DNA aberrations that would indicate point mutations or minor variants. NCI-H661 cells stain positive for keratin and vimentin fibers and negative for neurofilament triads.
Tissue	Lung
Disease	Carcinoma
Morphology	Epithelial
Gender	Male
Age	43 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer Research 2. Drug Development Research 3. Genomics and Proteomics research 4. Signal Transduction Studies 5. Immunotherapy Research 6. Gene Editing and Genetic Studies 7. Toxicology Studies
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	Researchers use NCI-H661 cells to perform various in vitro experiments to explore the cellular and molecular characteristics of lung cancer, to screen potential anti-cancer drugs, and to understand the tumor microenvironment and cancer progression mechanisms.
Characteristics
Karyotype	Hyperhexaploid; modal number = 142; range = 130 to 153. More than 70 marker chromosomes per cell.
Metastatic Site	Lymph node
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	The volumes used in this protocol are for 75 cm² flasks. For culture vessels of other sizes, the amount of dissociation medium should be proportionally reduced or increased. 1. Remove and discard the medium. 2. Briefly rinse the cell layer with 0.25% (w/v) trypsin - 0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 1.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to aspirate the cells. 5. Transfer the cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. 6. Discard the supernatant and resuspend the cells in fresh growth medium. Add the appropriate cell suspension to a new culture vessel. 7. Place the culture vessel in a 37℃ incubator.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	The ratio of 1:3 to 1:5 is recommended.
Culture Medium	RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37℃
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

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Cat.No.	Product Name	Price
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* For research use only. Not intended for any clinical use.

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