COLO 205 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	COLO 205 is a human colon cancer cell line originally isolated in 1957 from the ascites of a 70-year-old white male colon cancer patient who had been treated with 5-fluorouracil for several weeks. This cell line is characterized by its epithelial morphology and is frequently used in biomedical research focused on colorectal cancer, particularly related to cancer biology, drug response, and metastasis mechanisms. COLO 205 cells exhibit a hyperdiploid karyotype and are known to form moderately well-differentiated adenocarcinomas when xenografted into immunodeficient mice. COLO 205 cells express several key oncogenic and tumor suppressor pathways, making them a valuable model for pharmacological testing and cancer research. They are sensitive to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), making them suitable for apoptosis studies. In addition, these cells have been widely used to study the pharmacodynamics of various chemotherapeutic drugs, providing insights into the action and resistance mechanisms of colorectal cancer treatments.
Tissue	Colon
Disease	Colorectal adenocarcinoma
Morphology	Epithelial
Gender	Male
Age	70 years
Product Format	Frozen
Growth Mode	Adherent/suspension, loosely attached
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer research 2. Drug screening 3. Genetic studies 4. Biomarker discovery 5. Immunology research 6. Cell signaling
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	The COLO 205 cell line is derived from Dukes' type D tumor and expresses a 36,000 dalton cell surface glycoprotein. These cells were negative for CSAp expression and positive for keratin expression. The COLO 205 cell line is used to study SV-40 monkey virus and provides a suitable host for studying colon cancer. COLO 205 cells are tumorigenic in nude mice and were used to create a cell line-derived COLO 205 xenograft mouse model. The COLO 205 colon tumor model is an established model for studying the in vivo efficacy of 5-FU or bevacizumab.
Characteristics
Antigen Expression	The cells are positive for keratin by immunoperoxidase staining.
Isoenzymes	G6PD, B, PGM1, 1-2, PGM3, 1-2, 6PGD, A, ES-D, 1-2, PEP-D, 1
Karyotype	The stemline chromosome number is hypertriploid, and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) are commonly found in monosomy, disomy and trisomic condition, although most of these markers were monosomic.
Tumorigenic	Yes, in nude mice.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 2. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 10 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 4. Add an appropriate amount of cell suspension to the centrifuge tube containing floating cells. Centrifuge at 125 x g for 5 to 10 minutes. Resuspend in fresh medium and plate at appropriate passage ratio. 5. Incubate cultures at 37°C. NOTE: The volumes used in this protocol are for a 75 cm² flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium.
Medium Renewal	Every 2 to 3 days
Subcultivation Ratio	The ratio of 1:2 to 1:6 is recommended.
Culture Medium	RPMI 1640 + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%;Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO0174	Cas9 Stable Cell Line - COLO 205	Inquiry
CSC-RR0123	GFP Stable Cell Line-COLO 205	Inquiry
CSC-RR0373	Luc/GFP Reporter Cell Line - COLO 205	Inquiry

* For research use only. Not intended for any clinical use.

Quick Inquiry