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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | BeWo is a cell line that exhibits epithelial morphology and was isolated from the placenta of a patient with choriocarcinoma. The b30 subclone of these cells can be grown on permeable membranes in double chambers to form confluent cell layers, allowing the rate of nutrient entry into the cell from the apical surface and efflux from the basement membrane to be determined. BeWo cells secrete a variety of hormones, including human chorionic gonadotropin (hCG), human chorionic growth hormone (placental lactogen), and steroid hormones such as estrone, estriol, and estradiol. However, the levels of β-hCG and estradiol secreted by BeWo cells are lower than those of other choriocarcinoma cell lines, such as JEG-3. Following forskolin treatment, β-hCG secretion in BeWo cells is increased to levels similar to those observed in other choriocarcinoma cell lines. In addition, forskolin treatment also increases the levels of progesterone secreted by BeWo cells. These cells have been widely used as an in vitro model to study the uptake of various nutrients into the placenta, including glucose, amino acids, and iron. |
| Tissue | Placenta |
| Disease | Choriocarcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | Fetus |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Placental research 2. Drug transport and metabolism 3. Toxicology studies 4. Cancer research 5. Cell signaling and pathway analysis 6. Development of drug delivery systems |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | CK 7 and E-cadherin are molecular markers that are expressed by BeWo cells. VE-cadherin is found in BeWo cells and is enhanced upon treatment with forskolin. BeWo cells also express keratin and are positive for G6PD, B isoenzyme. |
| Characteristics | |
| Karyotype | The karyotype of BeWo cells is modal number = 86, with a range of 71 to 178, and the stemline number is hypotetraploid. |
| Genes Expressed | Progesterone; human chorionic somatomammotropin (placental lactogen); human chorionic gonadotropin (hCG); estrogen, (estrone; estriol; estradiol); keratin positive by immunoperoxidase staining |
| Isoenzymes | G6PD, B |
| Virus Susceptibility | Human poliovirus 3; Vesicular stomatitis virus |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The volumes given are for 75 cm2 flasks. For culture vessels of other sizes, increase or decrease the amount of dissociation medium required proportionally. 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently remove the cells by pipetting. 5. Add an appropriate amount of the cell suspension to a new culture vessel. 6. Incubate the culture at 37℃. |
| Medium Renewal | 3 to 4 times per week |
| Subcultivation Ratio | The ratio of 1:3 is recommended. |
| Culture Medium | Ham's F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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