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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The T84 cell line is a transplantable human cancer cell line derived from a lung metastasis of colon cancer in a 72-year-old male. T84 cells spontaneously differentiate into a structurally and functionally mature absorptive epithelial monolayer upon confluence. This maturation is complete over a period of 2-3 weeks and monolayer integrity is routinely assessed by measuring transepithelial electrical resistance (TEER). The differentiated phenotype is characterized by a columnar, polarized cell morphology, the formation of tight junctions separating the apical membrane region from the basal membrane region, and the presence of an apical brush border studded with microvilli. Therefore, differentiated T84 monolayers are a well-established in vitro model system for human intestinal epithelial cells and are frequently used to study drug absorption, metabolism, bioavailability, electrolyte transport, and the effects of compounds on epithelial barrier integrity. |
| Tissue | Colon |
| Disease | Carcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 72 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Biology Studies 2. Drug Discovery and Development 3. Genomic and Epigenomic Research 4. Immunotherapy Research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | T84 cell line exhibits tight junctions, and desmosomes between adjacent cells. The cells should be maintained at high density (at least 1/4 confluency). The cells are positive for keratin by immunoperoxidase staining. |
| Characteristics | |
| Karyotype | The stem line had a modal chromosome number of 56, with an incidence of 28% and a polyploidy rate of 12.4%. Most metaphase cells had a total of 18 markers. The normal X and 13 chromosomes were missing. Chromosomes 2, 4, and 22 were present in single copies, and chromosome 12 was present in 4 copies. |
| Tumorigenic | Yes, in nude mice. |
| Receptors Expressed | Peptide hormone, neurotransmitter |
| Antigen Expression | Keratin + (Immunoperoxidase staining) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old medium from adhered cells and wash with PBS without calcium and magnesium. For T25 flasks, use 3-5 ml PBS and for T75 flasks, use 5-10 ml. 2. Then completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to incubate at room temperature for 8-10 minutes to allow them to detach. 4. After incubation, gently mix the cells with 10 ml medium to resuspend, then centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells with fresh medium, and transfer them to a new flask already containing fresh medium. |
| Medium Renewal | Twice per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | Ham's F12 + DMEM (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
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