Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Quantitative PCR (qPCR) is a powerful and commonly used technique for the precise analysis of gene expression. Probe-based qPCR is a variant of the core qPCR technique that employs fluorescent probes to enable specific target detection during PCR amplification. A fluorescent probe is a short piece of DNA labeled with a fluorophore and a quencher. Initially, the quencher's proximity to the fluorophore prevents it from emitting fluorescence despite being excited by the light source. However, during amplification, the probe binds to the target sequence, is cleaved by the polymerase, and in the process separates the fluorophore from the quencher. This separation produces a fluorescent emission that correlates directly to the amplification of the target DNA.
Probe-based qPCR is commonly used in clinical and diagnostic applications, as it is well suited for any application requiring maximum specificity and consistent performance. Probe-based qPCR is able to achieve this level of performance because the probes are highly specific for the target sequence and the formation of primer-dimers is very rare. Since each probe is uniquely labeled, multiple targets can be amplified in the same tube by multiplexing. This increases throughput, minimizes sample handling, and potentially reduces reagent and consumable usage. Scientists can use these kits to analyze changes in the expression of one or more genes under different conditions or treatments. The high specificity conferred by probe-based techniques facilitates accurate and precise quantification, facilitating efficient comparisons between different samples.
Creative Biogene offers probe-based qPCR kits in several formats to meet your experimental needs and give you the flexibility to perform a variety of applications. When performing probe-based qPCR, specificity and sensitivity, ease of use, and process safety are important to the success of the experiment. Creative Biogene's qPCR kits for use with qPCR probes feature a unique combination of process controls, including built-in visual indicators, to eliminate variables and ensure precise reaction setup. Thanks to a novel hot-start mechanism, our kits exhibit high specificity and sensitivity, enabling you to detect as little as one copy of your target sequence.
If you are unsure which assay and reagents are right for your application, please review our qPCR specific product pages, or contact a member of our team for a recommendation.
| Cat.No. | Product Name | Price |
|---|---|---|
| QPHC-01 | qPCR ToughMix Kit with ROX (probe) | Inquiry |
| QPHC-02 | qPCR ToughMix Kit (probe) | Inquiry |
| QPOC-01 | TaqMan Fast Virus One-Step qRT-PCR Master Kit (Probe) | Inquiry |
| QPQC-01 | Fast qRT-PCR Surpermix Kit (Probe) | Inquiry |
| QPQC-02 | qPCR Master Mix Kit with ROX (Probe) | Inquiry |
Copyright © Creative Biogene. All rights reserved.