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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | RAW 264.7 cells are a widely used murine macrophage cell line derived from the ascites fluid of male mice with Abelson murine leukemia virus-induced tumors and are commonly used in immunology and infectious disease research. As an immortalized cell line, RAW264.7 cells are a key model system for studying macrophage biology, including immune responses to pathogens, signal transduction, and gene expression. RAW264.7 cells are particularly valuable for their ability to differentiate into macrophage-like cells. These cells can polarize into M1 macrophages, which are associated with inflammatory responses, or M2 macrophages, which are associated with tissue repair and anti-inflammatory processes. Under certain conditions, such as exposure to RANKL (Receptor Activator of Nuclear Factor κB Ligand), RAW264.7 cells can be induced to differentiate into osteoclast-like cells, making them a model for studying certain aspects of osteoclast biology and bone resorption. |
| Tissue | Ascites |
| Disease | Abelson murine leukemia virus-induced tumor |
| Morphology | Monocyte/macrophage |
| Age | Adult |
| Gender | Male |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Study of macrophage biology 2. Inflammation research 3. Infectious disease research 4. Drug discovery 5. Cancer research 6. Cytotoxicity testing 7. 3D cell culture |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The response of the RAW264.7 cell line to various stimuli, including induction of pyroptosis, an inflammatory cell death process triggered by factors such as LPS (lipopolysaccharide), helps dissect the pathways leading to the production of inflammatory cytokines. The impact of environmental conditions, such as extracellular glucose levels, on cell function and phenotype provides insights into potential downregulation of cellular metabolism and inflammatory responses. |
| Characteristics | |
| Receptors Expressed | Immunoglobulin (Fc), complement (C3) |
| Antigen Expression | H-2a |
| Viruses | Cell lines were tested and found to be positive for reverse transcriptase (RT) activity of type C retroviruses in cell culture supernatants and cell extracts. Micepox virus (mousepox) may be secreted. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old culture medium from adherent cells and wash with calcium- and magnesium-free PBS. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, resuspend cells by gently mixing with 10 ml of medium and centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | Replace or add medium every 2 to 3 days. |
| Subcultivation Ratio | The ratio of 1:3 to 1:6 is recommended. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
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