Plant Transformation Service

Creative Biogene has focused on routine plant tissue culture and genetic transformation to offer customers with high quality service over the past decade. We have transformed major agronomic crops successfully, such as rice, maize, cotton, wheat, barley, and model plants, such as Arabidopsis, tobacco. In addition, Creative Biogene's staffs have extensive experience in the development of transformation protocols for species for which transformation methodologies are not available or are currently inefficient. With the most advanced equipment and professional scientists, we will work closely with you to bring satisfactory plant transformation service for your research.

Plant genetic transformation is a way to insert foreign DNA into the plant genome. It is now a powerful tool in plant biology research and plays an important role for crop breeding and improvement. Agrobacterium-mediated T-DNA (transfer DNA) transformation, particle bombardment delivery and PEG-mediated protoplast transformation are the most popular delivery strategies applied widely in plant transformation at present. Which delivery strategy to be chose depends on the receptor species and specific research purpose. With the development of plant transformation technology, transgenic breeding has emerged. This method has thoroughly changed the production mode of plant variety.

Delivery Strategy	Advantages	Applicative Species
Agrobacterium-mediated transformation	Low copy numbers integration, stable inheritance, low cost.	Dicots, a few monocots.
Particle bombardment	Extensive receptor species, convenient operation.	Nearly all species.
PEG-mediated transformation	Fast, low cost.	Individual plants like tobacco, lettuce etc.

Experimental process of Creative Biogene:

Construction of expression vector
Callus induction and culture
Plasmids transfer into callus
Selection of transformed callus
Plant regeneration
Transgenic plant verification

Fig. 1 Agrobacterium-mediated Rice Transformation

Advantages of plant transformation in Creative Biogene:

Efficient and stable transformation system
Various delivery strategies for multiple species and genotypes
Short experimental period, just 3 to 9 months for routine plants
Years of experience in plant genetic transformation system

Creative Biogene provides plant transformation services for the plant species listed in the table below for clients around the world. We will offer seeds of positive T1 generation over 5 lines.

Species	Genotype	Selection System	Lines of Positive T1 Generation
Rice	Kitaake, Nipponbare	hygromycin	10
Maize	Hi-II A X B	hygromycin	10
Cotton	Coker 312, Acala B1654	hygromycin	10
Wheat	Fielder, Bobwhite	hygromycin, glufosinate	10
Barley	Golden Promise	hygromycin, glufosinate	10
Arabidopsis	Col, Ler, Ws	hygromycin	10
Tobacco	Benthamiana, Sr1, Samsun, TI1347, Xanthi	kanamycin, hygromycin, glufosinate	10
Tomato	Money maker, T-5, UC 82B, VF 36, MicroTom	kanamycin, hygromycin, glufosinate	10
Petunia	Mitchell diploid	kanamycin	10
Pepper	R&C Cayenne	glufosinate	5
Lettuce	most genotypes	kanamycin	10
Citrus	Carrizo	kanamycin	5
Canola	Westar	kanamycin	5
Alfalfa	Regen	kanamycin	10

For further information about our plant tissue culture or transformation service, please feel free to contact us.

Reference:

Wang, K. (2015). Preface.agrobacterium protocols. Methods in molecular biology, 1224, vii-viii.

* For research use only. Not intended for any clinical use.

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