Our promise to you:
Guaranteed product quality, expert customer support.
Creative Biogene has focused on routine plant tissue culture and genetic transformation to provide customers with high quality service over the past decade. We have transformed major agronomic crops successfully, such as rice, maize, cotton, wheat, barley, and model plants, such as arabidopsis, tobacco. In addition, the Creative Biogene's staff have extensive experience in the development of transformation protocols for species for which transformation methodologies are not available or are currently inefficient. With the most advanced equipment and professional scientists, we will work closely with you to bring satisfied plant transformation service for your research.
Plant genetic transformation is a way to insert foreign DNA into the plant genome. It is now a powerful tool in plant biology research and plays an important role for crop breeding and improvement. Agrobacterium-mediated T-DNA (transfer DNA) transformation, particle bombardment delivery and PEG-mediated protoplast transformation are the most popular delivery strategies applied widely in plant transformation at present. Which delivery strategy to be chose depends on the receptor species and specific research purposes. With the development of plant transformation technology, transgenic breeding has emerged. This method has thoroughly changed the production mode of plant variety.
|Delivery Strategy||Advantages||Applicative Species|
|Agrobacterium-mediated transformation||Low copy numbers integration, stable inheritance, low cost.||Dicots, a few monocots.|
|Particle bombardment||Extensive receptor species, convenient operation.||Nearly all species.|
|PEG-mediated transformation||Fast, low cost.||Individual plants like tobacco, lettuce etc.|
Some laboratories now face the problems of inefficiency and limited receptor species in plant genetic transformation. Creative Biogene dedicates the exploitation of efficient transformation systems and extensive receptor plants. We will work together with you closely and solve your problem.
Experimental process of Creative Biogene:
Fig 1. Agrobacterium-mediated Rice Transformation (Wang et al, 2015)
Advantages of plant transformation in Creative Biogene:
Creative Biogene provides plant transformation services for the plant species listed in the table below for clients around the world, we will offer seeds of positive T1 generation over 5 lines.
|Species||Genotype||Selection System||Lines of Positive T1 Generation|
|Maize||Hi-II A X B||hygromycin||10|
|Cotton||Coker 312, Acala B1654||hygromycin||10|
|Wheat||Fielder, Bobwhite||hygromycin, glufosinate||10|
|Barley||Golden Promise||hygromycin, glufosinate||10|
|Arabidopsis||Col, Ler, Ws||hygromycin||10|
|Tobacco||Benthamiana, Sr1, Samsun, TI1347, Xanthi||kanamycin, hygromycin, glufosinate||10|
|Tomato||Money maker, T-5, UC 82B, VF 36, MicroTom||kanamycin, hygromycin, glufosinate||10|
Wang, K. (2015). Preface. agrobacterium protocols. Methods Mol Biol,1224, vii-viii.