U-87 MG Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	U-87 MG (Uppsala 87 malignant glioma) is one of the most widely used human cell lines in brain cancer research. Originally established at Uppsala University in 1966, it originated from a malignant glioma in a 44-year-old male patient. These cells exhibit an epithelial-like morphology, growing in an adherent monolayer and often forming loose cell clusters. Although genomic analysis indicates differences between the widely distributed ATCC/ECACC commercial strains and the original Uppsala tumor, this cell line remains the gold standard model for studying the molecular mechanisms of glioblastoma multiforme (GBM) due to its well-defined growth pattern and high invasiveness.
Tissue	Brain
Disease	Glioblastoma; Astrocytoma
Morphology	Epithelial-like; Elongated; Polygonal
Gender	Male
Age	44 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1
Applications	1. Brain Cancer Research 2. Drug Discovery and Delivery 3. Immuno-oncology Studies 4. In Vivo Xenograft Modeling 5. Signal Transduction and Genomics
Shipped In	Dry ice
Storage Temperature	Below −140°C or in liquid nitrogen vapor phase
Characteristics
Karyotype	Hypodiploid; modal chromosome number = 44 (occurring in ~48% of cells).
Tumorigenic	Yes, highly tumorigenic in nude or SCID mice; forms well-vascularized tumors.
DNA Profile (STR)	Amelogenin: X; CSF1PO: 10, 11; D13S317: 8, 11; D16S539: 12; D5S818: 11, 12; D7S820: 8, 9; TH01: 9.3; TPOX: 8; vWA: 15, 17.
Mutations	Homozygous PTEN mutation/deletion; NF1 heterozygous mutation; TERT promoter mutation; typically IDH1 wild-type.
Markers Expressed	NKG2D ligands; ICAM-1 (enhanced by retinoic acid); GFAP expression (variable, often decreased in late passages).
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Gently rinse the cell layer with calcium- and magnesium-free PBS buffer to remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% (w/v) trypsin-0.53 mM EDTA solution (for T75 culture flasks). 4. Observe under an inverted microscope until the cell layer disperses (usually 5 to 15 minutes). Do not tap or shake the culture flask to avoid cell clumping. 5. Add 6.0 to 8.0 mL of complete culture medium and gently aspirate the cells using a pipette. 6. Centrifuge the cell suspension at 125 x g for 5 to 10 minutes. 7. Resuspend the cell pellet in fresh complete culture medium and aliquot into new culture containers.
Medium Renewal	2 to 3 times per week.
Subcultivation Ratio	A split ratio of 1:2 to 1:5 is recommended.
Culture Medium	EMEM (Eagle's Minimum Essential Medium) + 10% Fetal Bovine Serum (FBS) + 1 mM Sodium Pyruvate + 1% Non-Essential Amino Acids (NEAA).
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Cryopreservation	90% to 95% complete growth medium supplemented with 5% to 10% (v/v) DMSO.

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Cat.No.	Product Name	Price
CSC-RO01229	Cas9 Stable Cell Line - U-87 MG	Inquiry
CSC-RR00974	GFP/Luc Reporter Cell Line - U-87MG	Inquiry
CSC-RR01128	AP1-Luc/EF1α-mCherry Reporter Cell Line - U87MG	Inquiry
CSC-RR01129	ARE-Luc/EF1α-mCherry Reporter Cell Line - U87MG	Inquiry
CSC-RR01130	ISRE-Luc/EF1α-mCherry Reporter Cell Line - U87MG	Inquiry
CSC-RR01131	Myc-Luc/EF1α-mCherry Reporter Cell Line - U87MG	Inquiry
CSC-RR01132	NFκB-Luc/EF1α-mCherry Reporter Cell Line - U87MG	Inquiry
CSC-RR01133	p53-Luc/EF1α-mCherry Reporter Cell Line - U87MG	Inquiry
CSC-RR01134	STAT3-Luc/EF1α-mCherry Reporter Cell Line - U87MG	Inquiry
CSC-RR01135	IL6pro-Luc/EF1α-mCherry Reporter Cell Line - U87MG	Inquiry
CSC-RR0647	Luciferase Reporter Cell Line - U87MG	Inquiry

* For research use only. Not intended for any clinical use.

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