Adeno-associated virus (AAV) has been widely used by scientists to deliver exogenous genetic material into cultured cells or live animal models. AAV can infect both dividing and non-dividing cells. Multiple AAV serotypes (AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ) enable AAVs to efficiently infect with broad specificity.
In general, recombinant AAVs are engineered to be persisting in an extrachromosomal state, which could reduce the risk of mutation. Due to this feature, AAV DNA is lost through cell division but can be stable in non-dividing cells.
So far, there are two types of AAV systems: Conventional Single-Stranded Adeno-Associated Virus (ssAAV) and Self-Complementary Adeno-Associated Virus (scAAV).
Table1. Comparison of ssAAV and scAAV
|Genome DNA||Single-Stranded DNA||Double-Stranded DNA (Intra-Sequence Complementary)|
|State Within Cell||Episome||Episome|
|Packaging Limit||<4.7 kb||<2.4 kb|
|AAV Serotype||AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ||AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9, AAV-DJ/8 and AAV-DJ|