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Plasmid vector construction and host bacterium selection can have an impact on yield and quality, as well as on later production processes, including issues such as stability of plasmid passaging, different expression states in passaging stability, and the safety of the strain. Creative Biogene can provide many types of high-quality plasmid construction services. Based on our extensive experience in vector design and plasmid construction, we can provide optimized design services for any vector from scientific research to drug development applications to meet our clients' diverse plasmid production demands.
Creative Biogene's vector construction strategy is divided into four main parts: selection of replication origin, selection of resistance genes, selection of regulatory sequences, and selection of target gene.
In order to increase the yield of plasmid DNA, we usually select high copy plasmids containing Col EI replication origin.
When selecting resistance genes, we avoid the use of β-lactam antibiotics because a very small amount can cause strong allergic reactions in some populations.
We construct plasmid DNA with due consideration of regulatory sequences such as promoter and termination signals that control efficient and stable expression of target genes in eukaryotic cells.
We can select target genes for plasmid construction and carefully screen promoters and terminators to strictly control their biological activity according to the client's project requirements.
Design primers to amplify the target gene fragment from the cloning vector (or genome) according to the customer's production requirements. Select/process vectors and perform ligation of target genes to vectors to obtain target plasmids.
Linkage products are transformed into receptor cells, cloned and screened for positive bacteria (enzymatic digestion to detect positive clones).
Sequencing verification, alignment with gene sequences.
Optimize the design of existing plasmids to increase productivity based on the customer's production demands.
| Deliverables | Turnaround Time | Price |
| 2-3 weeks | Inquiry |
Creative Biogene provides efficient vector construction services to our customers. All custom vectors are subject to strict quality control and testing. We ensure that the delivered vectors meet the customer's design requirements. Please feel free to contact us if you have a need, and our experienced specialists will be happy to assist you.
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