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| General Information | |
| Organism | Rattus norvegicus, rat |
| Cell Line Description | The H9c2 (2–1) myoblast cell line, isolated from ventricular tissue, is currently used in vitro as a mimetic for skeletal and cardiac muscle due its biochemical, morphological and electrical/hormonal signaling properties. The H9c2 cell line was originally isolated from the ventricular portion of the BDIX rat heart. Thirteen days after fertilization, the cells were isolated and immortalized. An important feature of this embryonic cell line is its ability to differentiate from mononuclear myoblasts into myotubes, acquiring an elongated shape and orienting in a parallel manner, when cultured in a medium with low serum concentrations. During differentiation, the cells primarily acquire a skeletal muscle phenotype, as evidenced by cell type-specific differentiation markers such as myogenin and MyoD. |
| Disease | None Stated |
| Morphology | Myoblast |
| Tissue | Heart; Myocardium |
| Age | Embryo |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cardiac research 2. Pharmacological testing 3. Molecular biology studies 4. Toxicology studies 5. Metabolic research 6. Signal transduction pathway research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Ménard et al. demonstrated that the addition of all-trans retinoic acid (RA) to 1% serum culture medium induced a large number of cells to adopt an adult cardiomyocyte phenotype characterized by overexpression of the α-1 subunit of the L-type calcium channel. H9c2 cells did not display contractile activity even when differentiated. However, H9c2 cells and isolated neonatal cardiomyocytes responded similarly to several stimuli, including the development of a hypertrophic response. |
| Characteristics | |
| Genes Expressed | Myokinase; creatine phosphokinase; myosin |
| Expression Markers | Acetylcholine, expressed |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The volumes given are for 75 cm2 flasks. For culture vessels of other sizes, increase or decrease the amount of dissociation medium as needed proportionally. If the culture becomes confluent, the myoblast population will be rapidly depleted. To prevent loss of myoblasts, the culture should be subcultured before confluence, and the line should be re-cloned and selected for myoblasts periodically. 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer disperses (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to dissociate. Cells that are difficult to dissociate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently aspirate the cells. 5. Add an appropriate amount of cell suspension to a new culture vessel. 6. Incubate the culture at 37℃. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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