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Coronaviruses (CoV) are a group of viruses, such as Severe Acute Respiratory Syndrome (SARS), Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2 or 2019-nCoV), that cause respiratory tract infections in human.
Coronaviruses have four major structural proteins: spike, envelope, membrane, and nucleocapsid. The entry of the CoV into cells is initiated by binding of its spike envelope glycoprotein (S) to its complement host cell receptor. The SARS coronavirus (SARS-CoV or SARS-CoV-1), for example, infects human cells by attaching to the angiotensin-converting enzyme 2 (ACE2) receptor.
Figure 1. The life cycle of SARS-CoV-1.
Considering the recent outbreaks of coronavirus disease 2019 (COVID-19) caused by the newly emerged SARS-CoV-2, and the potential of future recurrence of SARS-CoV-1, development of effective and safe vaccines remains a high priority. Since CoV uses its spike glycoprotein (S) to bind its receptor and mediate virus entry, the S protein is a main target for antibody screening. CoV S glycoprotein pseudotyped virus is a useful tool for promoting the development of antibody screening. The S pseudotyped virus lacks infectivity and can be handled in BSL-2 facilities.
Creative Biogene offers CoV-S pseudotyped lentivirus product and custom service. Pseudotyped viruses expressing CoV S protein are generated by cotransfecting HEK293T cells with three plasmids: one containing a reporter gene such as GFP, one containing CoV S envelope protein encoding sequence and one containing gag/pol.
Figure 2. Infection of SARS-CoV-2 S pseudotyped GFP lentivirus to HEK293T-ACE2 cells.
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