LoVo Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	The LoVo epithelial cell line was isolated from a metastatic tumor nodule fragment in the left supraclavicular region of a 56-year-old Caucasian male patient with colorectal adenocarcinoma by Drewinko et al. in 1976. The LoVo cell line is a well-differentiated cell line that possesses most of the characteristics of enterocytes, including acinar structure, microvilli, glycocalyx, desmosomes, adhesions, and tight junction formation. LoVo has proven to be an effective tool for studying epithelioid tissue loss. LoVo cells were negative for colon antigen 3 and colon-specific antigen-p (CSAp-) expression. In addition, these cells express the N-ras, H-ras, Myb, c-myc, K-ras, sis, and fos oncogenes.
Tissue	Large intestine; Colon
Disease	Adenocarcinoma
Morphology	Epithelial
Gender	Male
Age	56 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	3D cell culture Cancer research Drug screening Immunotherapy research
Shipped in	Dry ice
Storage Temperature	−196°C
Additional Info	N-myc and sis oncogene expression was not detected. Expresses tumor-specific nuclear matrix proteins CC-3 and CC-4.
Characteristics
Karyotype	The number of chromosomes in the stem line was hyperdiploid, the occurrence rate of 2S components was about 2.7%, and there were a total of 3 marker chromosomes in all S metaphases. The karyotype is usually homogeneous and stable.
Oncogene	myc+; myb+; ras+; fos+; p53+; sis-; abl-; ros-; src-
Tumorigenic	Yes, in nude mice.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm² flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	The ratio of 1:3 to 1:10 is recommended.
Culture Medium	F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) contains 2 mM L-glutamine and 10% of fetal bovine serum.
Culture Conditions	Air: 95%; Carbon dioxide (CO₂): 5%; Temperature: 37℃
Cryopreservation	95% FBS + 5% DMSO (Dimethyl sulfoxide)

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO0038	Cas9 Stable Cell Line - LoVo	Inquiry
CSC-RR00791	Luciferase Reporter Cell Line - LOVO	Inquiry
CSC-RR0372	Luc/GFP Reporter Cell Line - LoVo	Inquiry
CSC-RR0485	GFP Reporter Cell Line - LOVO	Inquiry
CSC-RT2577	Human ATF1 Knockout Cell Line-LoVo	Inquiry
CSC-RT2654	Human OLA1 Knockout Cell Line-LOVO	Inquiry

* For research use only. Not intended for any clinical use.

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