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Long non-coding RNAs (lncRNAs), exceeding 200 base pairs without significant open reading frames, play diverse roles in genomic functions, including chromosome integrity, X chromosome inactivation, and epigenetic regulation. Aberrant lncRNA expression is implicated in tumorigenesis, making them potential therapeutic targets.
Creative Biogene offers high-quality lncRNA qPCR primer sets designed for gene-specific quantification assays. These ready-to-use products, competitively priced, facilitate efficient lncRNA research. Choose from our flexible primer sets by providing species and gene names, allowing personalized applications in your studies. Accelerate your research with our reliable and efficient tools.
Key Features of Our LncRNA qPCR Primer Sets
LncRNA qPCR Primer Sets can be applied across various research directions involving long non-coding RNAs (lncRNAs). These primers are suitable for studies exploring lncRNA functions in genomic maintenance, chromosome integrity, X chromosome inactivation, and epigenetic regulation. Additionally, they prove valuable in investigating aberrant lncRNA expression's role in tumorigenesis, offering insights into cancer research. The flexibility and specificity of these primer sets make them versatile tools for researchers studying diverse aspects of lncRNA biology and their potential therapeutic applications.
Case Study 1
Researchers utilized lncRNA RT-qPCR primer sets to identify a novel pathway linking environmental toxicants, AHR activation, and the expression of MALAT1 in pancreatic cancer cells and tissues. MALAT1, a long noncoding RNA, was found to interact with EZH2, enhancing its epigenetic silencing activity. The study demonstrated that AHR-mediated MALAT1 induction contributes to epigenetic alterations in pancreatic tissues and cancer cells. Interestingly, the use of AHR antagonists, CH223191 or resveratrol, counteracted MALAT1 induction and its enhancement of EZH2 activity. This research sheds light on the intricate AHR-MALAT1-EZH2 signaling axis, revealing a potential mechanism for environmental exposure-induced epigenetic changes in the context of pancreatic tissues and cancer cells.
Figure 1. Researchers employed lncRNA RT-qPCR primers, revealing MALAT1-EZH2 interaction and toxicant-induced MALAT1 in pancreatic cancer cells. (Lee JE, et al., 2020)
Case Study 2
Researchers utilized qPCR methods to explore the transcriptional dynamics of long noncoding RNAs (lncRNAs). Their allele-sensitive single-cell RNA sequencing revealed that lncRNAs exhibit a longer duration between two transcriptional bursts compared to messenger RNAs. The study also uncovered increased cell-to-cell variability in lncRNA expression due to lower frequency bursting, generating larger numbers of RNA molecules. Exploiting cellular heterogeneity, researchers identified and experimentally validated lncRNAs with cell state-specific functions related to cell cycle progression and apoptosis. Additionally, the study identified cis-functioning lncRNAs, demonstrating their impact on nearby protein-coding gene transcriptional bursting through knockdown experiments.
Figure 2. Researchers utilized qPCR to validate the relative expression levels of candidate cell cycle-regulated lncRNAs in lentiviral transduced NIH/3T3 cells. (Johnsson P, et al., 2022)
Q: How do lncRNA qPCR primer sets contribute to cancer research?
A: LncRNA qPCR primer sets aid cancer research by accurately quantifying aberrantly expressed long non-coding RNAs (lncRNAs) linked to tumorigenesis. This precision enables researchers to investigate the roles of these lncRNAs in cancer development, progression, and potential therapeutic strategies.
Q: In what areas of genomic research are lncRNA qPCR primer sets particularly useful?
A: LncRNA qPCR primer sets are valuable in genomic research areas such as genomic maintenance, chromosome integrity, X chromosome inactivation, and epigenetic regulation. They enable precise quantification of lncRNAs involved in these processes, enhancing understanding of genomic functions.
Q: What distinguishes lncRNA qPCR primer sets in terms of design and application?
A: LncRNA qPCR primer sets are meticulously designed for precision, offering ready-to-use convenience and sensitivity in detecting low-abundance lncRNAs. Additionally, they provide flexibility through customization options, ensuring alignment with researchers' specific experimental needs.
Q: Is support available for researchers using lncRNA qPCR primer sets?
A: Yes, comprehensive support is often provided, including assistance with customization based on research needs and personalized guidance. This ensures researchers have the necessary tools and support for successful long non-coding RNA studies.
| Cat.No. | Product Name | Price |
|---|---|---|
| HLQPS-1 | Human LncRNA qPCR Primer Sets | Inquiry |
| HLQPS-2 | All-in-one Human LncRNA qPCR Primer Sets | Inquiry |
| MLQPS-1 | Mouse LncRNA qPCR Primer Sets | Inquiry |
| MLQPS-2 | All-in-one Mouse LncRNA qPCR Primer Sets | Inquiry |
| RLQPS-1 | Rat LncRNA qPCR Primer Sets | Inquiry |
| RLQPS-2 | All-in-one Rat LncRNA qPCR Primer Sets | Inquiry |
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