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Engineered using CRISPR technology, these cell lines are rigorously verified via PCR and sequencing to confirm the double-knockout genotype. The resulting knockout cell lysate, prepared in RIPA buffer, includes a parental cell line lysate for internal control. Primarily serving as negative controls for western blots, these lysates validate antibody specificity, crucial for eliminating nonspecific binding. With potential applications as negative controls for functional assays, Creative Biogene's Knockout Cell Lysate series offers researchers a reliable solution for precise genotype confirmation and experimental validation.
Quality Advantages
knockout cell lines and their lysates serve as invaluable tools for quality control in various experimental assays. They provide researchers with reliable negative controls for western blotting, ensuring the specificity and accuracy of antibody detection. Moreover, these products facilitate the validation of drug targets by enabling the assessment of target protein function upon gene knockout. Furthermore, their application extends to biomarker discovery, where they aid in the identification and validation of biomolecules with diagnostic or prognostic significance. Specifically, knockout cell lines and their lysates are indispensable resources that contribute to the advancement of scientific research across diverse fields:
Case Study 1
Despite growing evidence of the importance of NAD+ levels for retinal health, the specific roles of subcellular NAD+ pools in retinal development, maintenance, and disease are still unclear. Researchers investigated the role of NMNAT1 in the mammalian retina and found that its deletion led to severe degeneration of photoreceptors and inner retinal neurons through various cell death pathways. To understand the underlying mechanisms, they analyzed disruptions in retinal central carbon metabolism, purine nucleotide synthesis, and amino acid pathways. Transcriptomic and immunostaining approaches also revealed dysregulation of photoreceptor and synapse-specific genes even before morphological or metabolic changes were detectable. These findings suggest that NMNAT1 plays a crucial role in maintaining retinal health and function, possibly by regulating gene expression during photoreceptor terminal differentiation.
Figure 1. Knockout Cell Lysate is utilized by researchers to evaluate the impact of gene knockout on cellular processes. It allows for the assessment of protein expression levels, such as NMNAT1, and enables the comparison of cellular morphology between knockout and control samples using techniques like Western blotting and histological analysis. (Sokolov D, et al., 2021)
Case Study 2
Prion diseases are deadly neurodegenerative disorders affecting both humans and animals, which can spread within and occasionally across species. Researchers investigated the impact of autophagy modulation on the release of prions packaged in exosomes and its implications for prion infection. Prions, known for causing fatal neurodegenerative diseases, can spread via exosomes present in bodily fluids. By studying murine neuronal cells, they found that stimulating autophagy inhibited exosomal prion release, while inhibiting autophagy increased exosome-mediated prion dissemination. Moreover, differences in basal autophagy levels between cell types influenced the amount of exosomal prion release. These findings highlight a novel role of autophagy in the prion life cycle, suggesting that manipulating autophagy could be a potential strategy for controlling prion diseases.
Figure 2. Researchers used CRISPR/Cas9 to create Atg5-deficient ScN2a cells (KO) lacking autophagy. Despite normal viability, Atg5-KO cells exhibited significantly higher exosome release compared to WT cells, with increased PrPSc in exosomes but unchanged levels in knockout cell lysates. This suggests that autophagy blockage enhances prion spread via exosomes. (Abdulrahman BA, et al., 2018)
Q: How does Creative Biogene ensure the quality and reliability of its Knockout Cell Lysate series?
A: We ensure the quality and reliability of our Knockout Cell Lysate series through several measures:
(1) Rigorous PCR and sequencing verification of the double-knockout genotype for each cell line.
(2) Uniform preparation of lysates in RIPA buffer, maintaining consistency across experiments.
(3) Inclusion of lysate from the parental cell line as an internal control, ensuring integrity in experimental comparisons.
Q: What are the potential applications of Creative Biogene's Knockout Cell Lysate series beyond western blotting?
A: Beyond western blotting, our Knockout Cell Lysate series holds potential for novel applications, including:
(1) Serving as negative controls for functional assays, ensuring specificity and accuracy in various experimental setups.
(2) Facilitating the validation of drug targets by assessing target protein function upon gene knockout, aiding in drug discovery efforts.
(3) Contributing to biomarker discovery by identifying and validating biomolecules with diagnostic or prognostic significance.
Q: How can researchers utilize Creative Biogene's Knockout Cell Lysate series in antibody validation studies?
A: Researchers can utilize our Knockout Cell Lysate series as an ideal platform for validating both polyclonal and monoclonal antibodies. These lysates ensure accuracy and reliability in antibody-based assays by providing reliable negative controls for specificity testing.
Q: In what ways can Creative Biogene's Knockout Cell Lysate series contribute to the study of cancer development and progression?
A: Our Knockout Cell Lysate series can significantly contribute to elucidating the underlying mechanisms driving cancer development and progression. By providing reliable tools for gene knockout studies, these lysates enable researchers to gain novel insights into tumor biology, paving the way for the development of innovative therapeutic strategies.
| Cat.No. | Product Name | Price |
|---|---|---|
| CLKO-0001 | VIM KO Cell Lysate-HeLa | Inquiry |
| CLKO-0002 | KRT7 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0003 | CD68 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0004 | KRT8 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0005 | CTNND1 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0006 | MSH6 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0007 | CCND1 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0008 | LGALS3 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0009 | VEGFA KO Cell Lysate-HeLa | Inquiry |
| CLKO-0010 | SDC1 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0011 | ERCC1 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0012 | MET KO Cell Lysate-HeLa | Inquiry |
| CLKO-0013 | IDH1 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0014 | CALD1 KO Cell Lysate-HeLa | Inquiry |
| CLKO-0015 | ALPP KO Cell Lysate-HeLa | Inquiry |
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