ARPE-19 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	The ARPE-19 cell line, derived from the retinal pigment epithelium (RPE) of a 19-year-old male, has functional characteristics similar to native RPE cells, making it a key epithelial cell model in ophthalmic research. When cultured in a 3D cell culture system or as cell monolayers on laminin-coated filters using low-serum medium, ARPE-19 cells achieve morphological polarization and form tight junctions, exhibiting behavior similar to that observed in vivo to the transepithelial resistance. These cells are used in studies related to the physiology of the vertebrate retina and retinal pigment epithelium. ARPE-19 cells express RPE-specific markers such as CRALBP and RPE-65 and are an excellent model for understanding the pigmentation process of the retinal pigment epithelium, including melanin synthesis and melanosome content.
Tissue	Eye, retinal pigmented epithelium, retina
Disease	Normal
Morphology	Epithelial
Gender	Male
Age	19 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	3D cell culture Modeling retinal function Disease modeling Drug screening Toxicity testing
Shipped in	Dry ice
Storage Temperature	−196°C
Additional Info	ARPE-19 is a spontaneously generated human RPE cell line with normal nucleology that forms polarized epithelial monolayers on porous filter supports. This cell line was established by selective trypsinization of primary RPE cultures, resulting in a homogeneous population of highly epithelial cells that exhibit robust growth potential.
Characteristics
Karyotype	Diploid
Antigen Expression	RPE-specific markers CRALBP and RPE-65
Tumorigenic	Yes
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Rinse the cell layer briefly with calcium- and magnesium-free PBS to remove all traces of serum containing trypsin inhibitors. 2. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 4. Add appropriate aliquots of cell suspension to new culture vessels. 5. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm² flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium.
Medium Renewal	Every 2 to 3 days
Subcultivation Ratio	The ratio of 1:3 to 1:5 is recommended.
Culture Medium	1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 2.5 mM L-glutamine, 0.5 mM sodium pyruvate and fetal bovine serum to a final concentration of 10%.
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%;Temperature: 37℃
Cryopreservation	95% FBS + 5% DMSO (Dimethyl sulfoxide)

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO01163	Cas9 Stable Cell Line - ARPE-19	Inquiry
CSC-RR00629	Luciferase Reporter Cell Line - ARPE-19	Inquiry
CSC-RT2730	Human ELMO1 Knockout Cell Line-ARPE-19	Inquiry
CSC-RT2736	Human PDGFRA Knockout Cell Line-ARPE-19	Inquiry

* For research use only. Not intended for any clinical use.

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