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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HCT-8 (also known as HRT-18) is an epithelial cell line isolated in 1977 from an ileocecal adenocarcinoma of a 67-year-old Caucasian male. It is the gold standard model for studying the biology of human colon cancer, particularly favored for its highly differentiated nature and ability to form tightly connected polarized monolayers of cells. HCT-8 cells are highly sensitive to a variety of chemotherapeutic agents and are often used to study drug resistance mechanisms. Notably, this cell line is also a susceptible host for many human viruses, including coronaviruses such as HCoV-OC43, making it a dual-purpose tool for both oncology and virology research. |
| Tissue | Colon; Ileocecum |
| Disease | Adenocarcinoma; Colorectal Cancer |
| Morphology | Epithelial |
| Gender | Male |
| Age | 67 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Colorectal cancer research and drug resistance studies 2. Viral infection and replication assays 3. Investigation of cell adhesion and epithelial barrier function 4. Drug screening and toxicity testing 5. 3D cell culture and tumor spheroid modeling |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Pseudodiploid; Modal number = 46 (ranges from 44 to 48) |
| Tumorigenic | Yes, in nude mice |
| Protein Expression | Carcinoembryonic antigen (CEA); Blood group A; Rh+; Keratin positive |
| Receptors Expressed | Epidermal Growth Factor Receptor (EGFR) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all serum residue. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the culture flask and observe under an inverted microscope until the cell layer disperses (usually within 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently aspirate the cells using a pipette. 5. Transfer an appropriate amount of cell suspension to a new culture vessel. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:4 to 1:10 is recommended |
| Culture Medium | RPMI 1640 supplemented with 10% Fetal Bovine Serum (FBS), 1 mM sodium pyruvate, and 2 mM L-glutamine. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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