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The utility of reporter genes encoding easily detectable proteins as markers for assessing transfection/transduction success, optimizing conditions, and studying gene expression. Creative Biogene provides adenovirus particles expressing various reporter genes including GFP, RFP, mCherry and luciferase, etc. These prepackaged adenoviruses are produced from our proprietary adenovirus expression platform which is based on human serotype 5 adenoviral backbone with E1 and E3 deletion. Our premade adenoviruses can be used as transduction controls to determine the optimal multiplicity of infection (MOI) during infection of your target cell. We also provide an in vivo grade of reporter-expressing adenoviral particles purified by ultracentrifugation.
Key Features of Our Reporter Adenovirus Particles
Reporter Adenovirus Particles Product List
Creative Biogene's versatile reporter adenoviruses are customizable tools for viral transduction biology. They enable real-time tracking of infection parameters, with GFP for uptake efficiency visualization and quantification, and Luciferase for highly sensitive bioluminescent readout. By cloning promoter-reporter fusions into adenoviral vectors, our approach offers insights into gene delivery kinetics. Explore the power of our products for:
Case Study 1
Genome-wide association studies (GWAS) on kidney function revealed risk regions, but understanding causal variants and mechanisms is limited. Using transcriptome-wide association studies (TWAS), researchers identified Dachshund homolog 1 (DACH1) as a critical gene associated with a GWAS risk variant linked to lower expression in human kidney tubules. Single-cell epigenome data pinpointed GWAS variants in specific kidney cells. CRISPR-Cas9 gene editing confirmed the role of risk variants in regulating DACH1. Mouse models demonstrated that DACH1 influences renal fibrosis, with its deletion exacerbating fibrosis and overexpression providing protection. Functional analysis revealed DACH1's impact on the cell cycle and chemotactic factors, contributing to macrophage infiltration and fibrosis. This integrated approach establishes DACH1 as a key gene in kidney disease risk.
Figure 1. Isolated primary mouse kidney tubule cells from Dach1fl/fl mice were infected with adenovirus-GFP (control) or adenovirus-Cre-GFP (Dach1 KO), confirming DACH1 protein depletion in Dach1 KO cells via Western blot analysis. GAPDH served as the loading control. (Doke T, et al., 2021)
Case Study 2
Ischemia or hemorrhage is characterized by a sudden reduction in blood volume and pressure due to blood vessel rupture, potentially leading to anoxic necrosis in multiple organs or even fatality. The researchers demonstrate that Mdivi-1, a selective inhibitor of Drp1, improves vascular reactivity in ischemic rats by attenuating oxidative stress. Mdivi-1's antioxidative effects, relatively Drp1-independent, involve increased antioxidant enzymes and enhanced Nrf2 expression. Mdivi-1 stabilizes hemodynamics, enhances organ perfusion, and improves rat survival, positioning it as a promising therapeutic agent for ischemic/hypoxic injury.
Figure. 2 Hypoxia-induced VSMCs transfected with GFP-Ad-shDrp1 showed robust inhibition of mitochondrial fission and a compensatory decrease in fusion. (Duan C, et al., 2020)
Case Study 3
Gliomas and meningiomas are the two most common types of human brain tumors. The researchers aimed to assess the anti-tumor effects of Ad/gTRAIL, an adenoviral vector expressing GFP and TRAIL under the control of the hTERT promoter, on malignant meningiomas and gliomas. Malignant meningiomas and gliomas lack effective cures, prompting the evaluation of Ad/gTRAIL's efficacy. The study involved infecting human malignant meningioma and glioma cells with Ad/gTRAIL and Ad/CMV-GFP. Proliferation assays, FACS analysis, and TRAIL quantification assessed cell viability and apoptosis.
Figure 3. Selective GFP-TRAI expression in malignant cells, not benign cells, post-Adv/g TRAIL infection, indicates tumor-specific promoter influence. No cytotoxicity in benign meningioma cells, but evident apoptosis in malignant glioma cells, consistent with the hTERT promoter's variable activity in different-grade brain tumors. (Li J T, et al., 2011)
Q: What is the mechanism of action for reporter adenovirus particles in delivering reporter genes to target cells?
A: Adenovirus particles use their natural infection mechanism to deliver the engineered reporter gene into target cells. The reporter gene is integrated into the adenovirus genome and expressed once inside the host cell.
Q: How do reporter genes function in the context of reporter adenovirus particles?
A: (1) Reporter genes act as markers for gene expression and are typically fused with a promoter region to regulate their activity.
(2) Examples of reporter genes include GFP for fluorescence, luciferase for bioluminescence, and β-galactosidase for colorimetric assays.
Q: What are the key advantages of using reporter adenovirus particles in molecular and cellular biology research?
A: (1) Efficient gene delivery: Adenoviruses have high transduction efficiency, ensuring effective delivery of the reporter gene.
(2) Versatility: Different reporter genes can be employed for various imaging and detection methods.
(3)In vivo applications: Reporter adenovirus particles are suitable for studying gene expression in living organisms.
Q: How are reporter adenovirus particles engineered, and what considerations are taken into account during their design?
A: (1) Engineering involves inserting the reporter gene into the adenovirus genome and ensuring proper regulatory elements (promoters).
(2) Considerations include selecting a suitable promoter, optimizing viral packaging, and ensuring compatibility with target cells.
Q: What are some common experimental applications of reporter adenovirus particles, and how do they contribute to molecular and cellular studies?
A: (1) Gene expression studies: Visualizing and quantifying gene expression patterns.
(2) Promoter analysis: Assessing the activity of specific gene promoters.
(3) Cellular imaging: Studying dynamic cellular processes in real-time.
(4) Drug screening: Evaluating the impact of compounds on gene expression.
(5)In vivo studies: Monitoring gene expression in living organisms.
| Cat.No. | Product Name | Price |
|---|---|---|
| AD00007Z | LacZ adenoviral particles | Inquiry |
| AD00281Z | GFP adenoviral particles | Inquiry |
| AD00330Z | Syn-GFP adenovirus | Inquiry |
| AD00332Z | EYFP-Actin adenovirus | Inquiry |
| AD00335Z | Luc adenovirus | Inquiry |
| AD00357Z | Syn-mCherry adenovirus | Inquiry |
| AD00358Z | mCherry adenovirus | Inquiry |
| AD00360Z | mRFP adenovirus | Inquiry |
| AD00376Z | Syn-RFP adenovirus | Inquiry |
| AD18650Z | E1(+)/GFP Human Adenovirus (Serotype 5) | Inquiry |
| AD18651Z | E1(+)/mCherry Human Adenovirus (Serotype 5) | Inquiry |
| AD18652Z | Ad5/F35-GFP Chimeric Adenovirus | Inquiry |
| AD18653Z | Ad5/F35-mCherry Chimeric Adenovirus | Inquiry |
| AD18654Z | Ad5/RGD-GFP Chimeric Adenovirus | Inquiry |
| AD18655Z | Ad5/RGD-mCherry Chimeric Adenovirus | Inquiry |
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