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Recombinant adenoviruses are efficient tools for gene delivery in mammalian cells, widely used in both in vivo and in vitro applications. They infect various cell types with high efficiency, and their transient extrachromosomal state ensures safety without genomic integration. Adenovirus DNA undergoes transcription, allowing high-level gene expression in most mammalian cells. Creative Biogene offers a diverse premade adenovirus collection with full-length ORFs, tags, and reporters for comprehensive gene studies. If your gene of interest is not in our collection, our customized adenoviral service caters to specific project needs, exemplifying our leadership in adenoviral technology.
Advantages of Our Gene-Specific Recombinant Adenoviruses
Gene-Specific Recombinant Adenoviruses Product List
The gene-specific adenoviral vectors enable simple and rapid functional studies by efficiently transducing target cells in vitro or in vivo to induce robust transient overexpression. The principle involves cloning full-length ORFs into adenoviral transfer vectors optimized for high gene delivery. By circumventing plasmid cloning and transfection, the ready-to-use adenoviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts. We provide powerful customizable tools for diverse functional genomics applications：
Case Study 1
Immune-checkpoint (IC) modulators, such as PVR and PD-L1, are potential targets in cancer therapy, including triple-negative breast cancer (TNBC). The researchers reveal that RB loss and high E2F1/E2F2 signatures correlate with PVR, CD274 (PD-L1 gene), and other IC modulators in TNBC cells. pRB represses, while RB depletion and E2F1 induce PVR and CD274. The CDK4/6 inhibitor palbociclib suppresses PVR and PD-L1 expression. Hydrochloric acid counteracts palbociclib's effect, inducing PD-L1 expression. Lactic acid, a glycolysis by-product, also induces PD-L1 and PVR. These findings propose a CDK4/6-pRB-E2F pathway regulating PD-L1 turnover, linking cell proliferation with immunomodulation and suggesting implications for cancer therapies.
Figure 1. TNBC cells were transduced with Ad.E2F1 or subjected to RB knockdown via RNAi. Western blot analysis confirmed that E2F1 overexpression induced both non-glycosylated (ngPD-L1) and glycosylated (gPD-L1) PD-L1 in RB-proficient and deficient cell lines. (Shrestha M, et al., 2023)
Case Study 2
Impaired mitochondrial fusion, driven by reduced mitofusin 2 (Mfn2) expression, contributes to uncontrolled proliferation and apoptosis resistance in diseases like pulmonary arterial hypertension (PAH) and non-small cell lung cancer (NSCLC). The study proposes that decreased Mfn2 levels result from increased proteasomal degradation triggered by phosphorylation at serine 442 (S442). PD-Mfn2 gene therapy showed greater tumor regression than WT-Mfn2 in an NSCLC xenotransplantation model, suggesting potential therapeutic benefits in PAH and lung cancer.
Figure 2. The two adenoviruses with mutated Mfn2 were designated Adv-Mfn2-S442A (PD-Mfn2) and Adv-Mfn2-S442D (CP-Mfn2). Adenoviral infection was carried out by infection with Adv-WT-Mfn2, Adv-PD-Mfn2 or Adv-CP-Mfn2 or adenovirus expressing bacterial-galactosidase (Adv-LacZ) as a control. (Dasgupta A, et al., 2021)
Q: What is the primary purpose of Gene-Specific Recombinant Adenoviruses in genetic research?
A: Gene-specific recombinant Adenoviruses are engineered to deliver and express specific genes of interest in a targeted and controlled manner within cells or organisms.
Q: How are these viruses customized for gene-specific applications?
A: Customization: These adenoviruses can be tailored to carry and express specific genes or genetic constructs, ensuring precise and efficient gene delivery.
Q: What advantages do Gene-Specific Recombinant Adenoviruses offer over other gene delivery methods?
A: These viruses demonstrate high transduction efficiency, ensuring effective gene delivery into a wide range of cell types. Gene-specific recombinant Adenoviruses allow for selective targeting of particular genes, minimizing off-target effects.
| Cat.No. | Product Name | Price |
|---|---|---|
| AD00043Z | Human E2F1 adenoviral particles | Inquiry |
| AD00044Z | Human hTERT adenoviral particles | Inquiry |
| AD00045Z | Human GPR6 adenoviral particles | Inquiry |
| AD00046Z | Human DRD1 adenoviral particles | Inquiry |
| AD00047Z | Human DRD2 adenoviral particles | Inquiry |
| AD00048Z | Human Claudin 1 adenoviral particles | Inquiry |
| AD00049Z | Human KCNK2 adenoviral particles | Inquiry |
| AD00050Z | Human murf-1 adenoviral particles | Inquiry |
| AD00051Z | Human murf-2 adenoviral particles | Inquiry |
| AD00052Z | Human murf-3 adenoviral particles | Inquiry |
| AD00053Z | Mouse LipG adenoviral particles | Inquiry |
| AD00054Z | Mouse AKAP8 adenoviral particles | Inquiry |
| AD00055Z | Human AKT1 adenoviral particles | Inquiry |
| AD00056Z | Human AKT2 adenoviral particles | Inquiry |
| AD00057Z | Human AKT3 adenoviral particles | Inquiry |
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