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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | OCM1A is a human uveal melanoma cell line derived from a primary intraocular tumor. Uveal melanoma is the most common primary intraocular malignancy in adults, and OCM1A serves as a critical model for studying the biology of this specific cancer. The cell line exhibits a spindle-shaped or epithelial-like morphology and is characterized by its rapid growth and high metabolic activity. OCM1A is frequently used to investigate the molecular signaling pathways involved in ocular tumor progression, particularly those related to G-protein mutations (GNAQ/GNA11) and the MAPK pathway. It is also a valuable tool for testing the efficacy of targeted therapies and photodynamic therapies specifically designed for eye cancers. |
| Tissue | Eye; Choroid/Uvea |
| Disease | Uveal Melanoma; Ocular Melanoma |
| Morphology | Spindle-shaped; Epithelial-like |
| Gender | Female |
| Age | 71 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Ocular oncology and uveal melanoma progression research 2. Study of MAPK and PI3K/Akt signaling pathways in eye cancer 3. Drug screening for targeted molecular therapies and chemotherapeutics 4. Investigation of tumor cell migration and invasion in the ocular microenvironment 5. Evaluation of radiotherapy and photodynamic therapy (PDT) sensitivity |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Genetic Profile | BRAF mutation (V600E); GNAQ/GNA11 wild-type; BAP1 wild-type |
| Expression Markers | Positive for Melan-A (MART-1) and S-100 protein |
| Melanogenic | Low to moderate melanin production |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with PBS (without Ca2+/Mg2+) to remove all traces of serum. 3. Add 1.0 to 2.0 mL of 0.25% Trypsin - 0.53 mM EDTA solution and observe under an inverted microscope until the cell layer is dispersed (usually 3 to 8 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to obtain a single-cell suspension. 5. Aliquot cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:8 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00816 | RFP Reporter Cell Line - OCM1A | Inquiry |
| CSC-RR00817 | Luciferase Reporter Cell Line - OCM1A | Inquiry |
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