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Bioinformatics

protocols
Protocol

ATAC-seq Protocol

Experiment Tags: ATAC-seq, Robust Tn5 Transposase, Tn5 Transposase

Experiment Summary: Sequence the library and correlate reads with open and closed chromatin. When sequencing ATAC-seq libraries, sequencing primers and reagents must be used.

protocols
Protocol

Fosmid Library Construction Protocol

Experiment Tags: Fosmid Library, Library, Library Construction

Experiment Summary: The fosmid system has been widely used in the construction of large-insert genomic libraries. Fosmid clones are similar in insert size comparing with cosmid clones and smaller than that in BAC and yeast artificial chromosomes (YAC).

protocols
Protocol

cDNA Library Construction Protocol

Experiment Tags: cDNA Library, Library, Library Construction

Experiment Summary: To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA).

protocols
Protocol

BCA Library Construction Protocol

Experiment Tags: BCA Library, Library, Library Construction

Experiment Summary: The size-fractionated DNA is cloned into a BAC vector and transformed into E. coli by electrical shock. The transformants are arrayed into microtiter dishes and high-density replica filters are prepared to facilitate screening of the library.

protocols
Protocol

Labeling Protocol for Microarray Analysis

Experiment Tags: Labeling, Microarray Analysis

Experiment Summary: This experiment describes how to perform labeling procedure for microarray analysis.