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Bioinformatics

protocols
Protocol

Single Guide RNA Library Design and Construction Protocol

Experiment Tags: Single Guide RNA Library, Library Design and Construction

Experiment Summary: This protocol describes how to generate a single guide RNA (sgRNA) library for use in genetic screening.

protocols
Protocol

CRISPRCas9 Library Screening Protocol

Experiment Tags: CRISPRCas9 Library Screening, CRISPRCas9

Experiment Summary: In this protocol, we briefly describe the CRISPR/Cas9 library screening process to simplify and facilitate high-throughput screening.

protocols
Protocol

Protocol for Constructing Glycine Oxidase Mutant Libraries by Random Mutagenesis

Experiment Tags: Glycine Oxidase Mutant Libraries, Random Mutagenesis

Experiment Summary: This protocol describes the construction of a glycine oxidase mutant library by random mutagenesis and the use of a phage T7 lysis-based approach for high-throughput screening of oxidase activity, among other things.

protocols
Protocol

Preparation of Genomic Overexpression Library Protocol

Experiment Tags: Genomic, Overexpression, Library

Experiment Summary: This protocol might work for searching complementation of other gene deletion mutants that have a good selective phenotype.

protocols
Protocol

Labeling Protocol for Microarray Analysis

Experiment Tags: Labeling,Microarray Analysis

Experiment Summary: This experiment describes how to perform labeling procedure for microarray analysis.

protocols
Protocol

BCA Library Construction Protocol

Experiment Tags: BCA Library, Library, Library Construction

Experiment Summary: The size-fractionated DNA is cloned into a BAC vector and transformed into E. coli by electrical shock. The transformants are arrayed into microtiter dishes and high-density replica filters are prepared to facilitate screening of the library.

protocols
Protocol

cDNA Library Construction Protocol

Experiment Tags: cDNA Library, Library, Library Construction

Experiment Summary: To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA).

protocols
Protocol

Fosmid Library Construction Protocol

Experiment Tags: Fosmid Library, Library, Library Construction

Experiment Summary: The fosmid system has been widely used in the construction of large-insert genomic libraries. Fosmid clones are similar in insert size comparing with cosmid clones and smaller than that in BAC and yeast artificial chromosomes (YAC).

protocols
Protocol

ATAC-seq Protocol

Experiment Tags: ATAC-seq, Robust Tn5 Transposase, Tn5 Transposase

Experiment Summary: Sequence the library and correlate reads with open and closed chromatin. When sequencing ATAC-seq libraries, sequencing primers and reagents must be used.

* For research use only. Not intended for any clinical use.
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