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ASO in vitro Screening Service


Creative Biogene is a leading biotechnology company which has the expertise and ability in providing antisense oligonucleotide (ASO) in vitro screening...

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ASO in vitro Screening Service

Creative Biogene is a leading biotechnology company which has the expertise and ability in providing antisense oligonucleotide (ASO) in vitro screening service for customers worldwide. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to provide you with the comprehensive ASO in vitro screening service, including in vitro ASO efficacy screening and ASO specificity analysis. Creative Biogene’s goal is to provide you with the most affordable, high-quality ASO in vitro screening service to ensure your satisfaction in a timely and professional manner.

Antisense oligonucleotide (ASO) is a short strand of deoxyribonucleotide analogue that hybridizes with the complementary mRNA in a sequence-specific manner via Watson–Crick base pairing. Formation of the ASO–mRNA heteroduplex either triggers RNase H activity, leading to mRNA degradation, induces translational arrest by steric hindrance of ribosomal activity, interferes with mRNA maturation by inhibiting splicing or destabilizes pre-mRNA in the nucleus, resulting in down-regulation of target protein expression. The ASO is not only a useful tool for studies of loss-of-gene function and target validation in experimental settings, but also highly valuable as a novel therapeutic strategy to treat any disease that is linked to dysregulated gene expression.

Fig. 1 Modes of action of antisense oligonucleotides (ASOs). [1]

Crucial issues to both experimental and therapeutic applications of ASO are the antisense oligonucleotides' efficiently and specificity. While some studies have shown that ASOs can discriminate differences in target RNAs as small as a single base change, other studies have suggested that oligonucleotides can have biological effects that are not attributable to specific degradation or blockade of their target RNAs. These effects can be due to sequence specific aptameric effects of oligonucleotides, to non-sequence specific binding of oligonucleotides to proteins, or to RNA cleavage due to partial sequence matches. Therefore, it is important to analyze the efficiency and specificity of ASOs.

Creative Biogene as one of the leading biotechnology company in the world is able to provide the following ASO in vitro screening service for your scientific research:

ASO efficacy screening

ASOs which in absence of efficient design algorithms need to perform an experimental screening to identify efficient ASO against the RNA target of interest. Creative Biogene possesses the state-of-the-art high-throughput workflows which can simultaneously tests hundreds of ASOs in vitro and finally selects candidate ASOs that knockdown of the target RNA in a dose-responsive manner.

ASO specificity analysis

Similar to all drugs, ASOs therapeutics also carries the risk of causing unintended toxicities or side effects. Knowing potential off-target related toxicity and side effects, allows you to further screens the candidate ASOs. Creative Biogene have the advanced assessment and experimental validation platform which can maps ASOs off-target effects of your candidate ASOs.

References:

  1. Chan JH & Lim S (2006). Antisense oligonucleotides: from design to therapeutic application. Clinical and experimental pharmacology and physiology,33(5‐6), 533-540.
  2. Matveeva O & Felden B (1998). Prediction of antisense oligonucleotide efficacy by in vitro methods. Nature biotechnology, 16(13), 1374.
  3. Lindow M & Bartz S (2012). Assessing unintended hybridization-induced biological effects of oligonucleotides. Nature biotechnology, 30(10), 920.

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