NCI-H292 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	NCI-H292 (also known as H292 or HuT-292) is a human epithelial cell line initially established in 1980 from lymph node metastases of pulmonary mucoepidermoid carcinoma. The donor was a 32-year-old woman. These cells were isolated in chemically defined culture media (HITES) and subsequently adapted to serum-added media. Biologically, NCI-H292 cells are significant because they retain characteristics of mucoepidermoid carcinoma in culture, including squamous differentiation and abundant small granules containing mucin. They are known to support the replication of various human viruses, particularly hepatitis B virus (HBV) and various paramyxoviruses, making them a dual-purpose model for oncology and infectious disease research.
Tissue	Lung
Disease	Carcinoma; Mucoepidermoid Pulmonary Carcinoma
Morphology	Epithelial; Polygonal
Gender	Female
Age	32 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Virology and Virus Replication Studies 2. Respiratory and Airway Research 3. Drug Screening and Development 4. Oncology and Functional Genomics
Shipped In	Dry ice
Storage Temperature	Below −140°C or in liquid nitrogen vapor phase
Characteristics
Karyotype	Near-diploid; Modal chromosome number is 47 (occurring in 36% of cells). Twelve marker chromosomes are common to most cells. Normal N1 and N6 are absent; two normal X chromosomes are present.
Tumorigenic	Yes, in nude mice; the resulting tumors retain mucoepidermoid histological features.
Mutations	The presence of a CRTC1-MAML2 (MECT1-MAML2) gene fusion from t(11;19)(q21;p13); heterozygous NF2 mutation; and homozygous TP53 mutation.
Markers Expressed	Keratin and vimentin were positive; MUC5AC mucin was highly expressed. L-DOPA decarboxylase and neurofilament triad protein were negative.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Gently rinse the cell layer with calcium- and magnesium-free PBS buffer to remove residual serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of 0.25% (w/v) trypsin-0.53 mM EDTA solution (for T75 culture flasks) and observe under an inverted microscope until the cell layer disperses (usually 5 to 15 minutes). For cells that are difficult to disperse, incubate at 37°C. 4. Add 6.0 to 8.0 mL of complete culture medium to neutralize the trypsin. 5. Gently pipette the cells and transfer them to centrifuge tubes. 6. Centrifuge at 125 x g for 5 to 10 minutes. 7. Resuspend the cell pellet in fresh complete culture medium and aliquot into new culture containers.
Medium Renewal	Every 2 to 3 days.
Subcultivation Ratio	A split ratio of 1:3 to 1:8 is recommended.
Culture Medium	RPMI-1640 Medium supplemented with 10% Fetal Bovine Serum (FBS) and 2 mM L-glutamine. Some variants utilize RPMI-1640 with 4500 mg/L glucose.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Cryopreservation	90% to 95% complete growth medium supplemented with 5% to 10% (v/v) DMSO.

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* For research use only. Not intended for any clinical use.

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