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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | A2780 is one of the most commonly used human ovarian cancer cell lines in biomedical research. It is derived from tumor tissue from untreated ovarian cancer patients. This cell line has an epithelial cell morphology and grows as an adherent monolayer in culture. The A2780 cell line is highly valued for its high sensitivity to various chemotherapeutic drugs, especially cisplatin and carboplatin. Over time, several chemotherapeutic resistant sublineages (such as A2780-CP and A2780-ADR) have been derived from this parent cell line, making it a cornerstone model for studying ovarian tumor drug resistance mechanisms, DNA repair, and the development of novel targeted therapies. |
| Tissue | Ovary |
| Disease | Ovarian Carcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | Not specified |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Ovarian cancer biology and signaling pathway research 2. Screening for novel platinum-based and taxane chemotherapies 3. Investigation of DNA damage and repair mechanisms 4. Study of drug resistance acquisition and reversal 5. Development of human tumor xenograft models in nude mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Genetic Profile | Wild-type p53; PIK3CA mutation; PTEN null |
| Expression Markers | Positive for epithelial markers (e.g., Cytokeratins) |
| Sensitivity | Highly sensitive to Cisplatin, Adriamycin, and Melphalan in its parental state |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca2+/Mg2+-free PBS buffer to remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution and observe under a microscope until the cell layer disperses (usually 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently pipette until the cell suspension is a single-cell suspension. 5. Aliquot the cell suspension into new culture containers. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:10 is recommended |
| Culture Medium | RPMI 1640 supplemented with 10% Fetal Bovine Serum (FBS), 2 mM L-glutamine, and 1% Penicillin-Streptomycin. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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