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DNA polymerase is an enzyme that catalyzes the polymerization of deoxyribonucleotides into DNA. It is present in all living organisms and participates in a variety of biological processes. These enzymes are essential for DNA replication and usually work in pairs to create two identical DNA strands from one original DNA molecule. During this process, DNA polymerase "reads" the existing DNA strands, creating two new strands that match the existing DNA strands. DNA polymerases are also involved in DNA repair, recombination and translesion synthesis.
There are many types of DNA polymerases, each with a different function. For example, DNA polymerase α is involved in the initiation and elongation of DNA replication, while DNA polymerases δ and ε are responsible for most of the DNA synthesis during replication. DNA polymerase β is involved in DNA repair while DNA polymerase γ is responsible for the replication of mitochondrial DNA.
DNA polymerase works by adding new nucleotides to the 3' end of an existing DNA strand. This reaction is a phosphoryl transfer reaction. The 3'-OH group of the growing chain acts as a nucleophile, attacking the incoming deoxyribonucleoside triphosphate at the α-phosphate, resulting in the formation of a phosphodiester bond. Inorganic phosphate is liberated in the reaction. It uses the nucleotide sequence of the template strand to determine which nucleotide to add next. The nucleotides introduced must be complementary to the bases on the template strand, as dictated by the base pairing rules (A to T, C to G). Once bound at the primer-template junction, DNA polymerase begins adding nucleotides to the 3' end of the primer. It does this by catalyzing the formation of a phosphodiester bond between the 3' hydroxyl group of the previous nucleotide in the DNA strand and the phosphate group of the incoming nucleotide. This process continues until the entire template chain has been replicated.
There are several reasons for choosing a different DNA polymerase, including improving fidelity, minimizing error rates in the amplification of long sequences, and improving speed. Enzyme selection is an important part of a successful PCR setup. Backed by years of manufacturing experience, Creative Biogene's DNA polymerases are unmatched in quality and purity. Every lot of our DNA polymerases undergoes rigorous quality control testing to ensure enzyme thermostability, elongation, processivity, and fidelity for the toughest PCR challenges.
If you are unsure which reagents are right for your application, please review our DNA Polymerase specific product pages, or contact a member of our team for a recommendation.
| Cat.No. | Product Name | Price |
|---|---|---|
| EMDT0316 | Hot Start Taq DNA Polymerase | Inquiry |
| EMDT0317 | Human DNA Polymerase Alpha | Inquiry |
| EMDT0318 | Human DNA Polymerase Beta | Inquiry |
| EMDT0319 | Human DNA Polymerase Gamma | Inquiry |
| EMDT0323 | Mako DNA Polymerase (3'-5' exo- ) | Inquiry |
| EMDT0324 | Manta 1.0 DNA Polymerase | Inquiry |
| EMDT0331 | Poly A Polymerase | Inquiry |
| EMDT0346 | Tth DNA Polymerase | Inquiry |
| EMDTS0010 | Taq DNA Polymerase (for PAGE) | Inquiry |
| EMDTS0012 | Taq Plus DNA Polymerase | Inquiry |
| EMDTS0013 | Taq Platinum DNA Polymerase | Inquiry |
| EMDTS0014 | Hotmaster Taq DNA Polymerase | Inquiry |
| EMDTS0015 | Long Taq DNA Polymerase | Inquiry |
| EMDTS0016 | Goldstar Taq DNA Polymerase (5U/uL, GoldStar Taq PCR Buffer with Mg2+) | Inquiry |
| EMDTS0017 | Goldstar Taq DNA Polymerase (2.5 U/uL,Taq Buffer, Mg2+) | Inquiry |
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