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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | 5637 is a cell line that exhibits epithelial morphology and was isolated from the bladder of a 68-year-old Caucasian male patient with grade II carcinoma. The karyotype of 5637 cells has a modal chromosome number of 67, ranging from 59 to 71. 5637 cells produce and secrete multiple growth factors such as SCF, IL-1, IL-6, G-CSF, and GM-CSF. 5637 cells are tumorigenic and have been shown to induce tumors in nude mice inoculated subcutaneously. Oncogene-wise, 5637 cells are positive for FGFR3, PIK3CA, HRAS, KRAS, NRAS, TERT, and CDKN2A, but negative for TP53, belonging to the molecular bladder cancer subtype luminal. 5637 cells are a valuable tool in cancer research, especially in the study of growth factors, cell division, oncogenes, and bladder cancer. |
| Tissue | Urinary bladder |
| Disease | Grade II Carcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 68 days |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. 3D cell culture 3. Gene expression and regulation 4. Drug screening and development |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The isoenzyme profile of 5637 cells consists of isoform 1 of AK-1, ES-D, Me-2 and PGM1, isoform 1 and 2 of GLO-I, isoform B of G6PD, as well as isoform 2 of PGM3. |
| Characteristics | |
| Karyotype | Modal chromosome number = 67; range = 59 to 71. The stemline modal chromosome number is 67 occurring at 36%, and polyploidy at 0.6%. |
| Isoenzymes | Me-2, 1, PGM3, 2, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. First, remove the old medium from the adherent cells and wash with PBS without calcium and magnesium. For T25 flasks, use 3-5 ml PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Let the cells incubate at room temperature for 8-10 minutes to allow them to detach. 4. After incubation, gently mix the cells with 10 ml of medium to resuspend, then centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells with fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | 2 to 3 times weekly |
| Subcultivation Ratio | The ratio of 1:4 to 1:8 is recommended. |
| Culture Medium | 90% RPMI 1640 + 10% h.i. FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don’t find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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