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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | The H22 cell line is a murine hepatocellular carcinoma cell line derived from liver tumor cells. H22 cells exhibit typical characteristics of hepatocellular carcinoma, including rapid proliferation, resistance to apoptosis, and the ability to form tumors when injected into appropriate animal models. Because these cells are derived from a mouse model, they are particularly suitable for studying the interaction between cancer cells and the immune system in a controlled environment. Researchers have utilized H22 cells to evaluate the efficacy of various immunotherapeutic agents, including checkpoint inhibitors and cancer vaccines. H22 cells are also used to study the role of liver-specific metabolic pathways and genetic mutations in the progression of hepatocellular carcinoma. In addition, the H22 cell line can be used to create the CDX (cell line derived xenograft) H22 xenograft mouse model. Studies have shown that the orthotopic H22 model increases immune cell populations of regulatory T cells and myeloid-derived suppressor cells in the bone marrow, spleen, and tumor tissue. |
| Tissue | Liver |
| Disease | Hepatocellular carcinoma |
| Morphology | Lymphoblast |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug screening and development 3. Immunotherapy studies 4. Gene expression analysis 5. Toxicology studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The H22 syngeneic mouse model consists of H22 mouse cells injected into a host with a fully functioning immune system (e.g. Balb/c). The H22 syngeneic model is used in studies focused on checkpoint inhibitors (e.g. anti-PDL-1 or anti-CTLA-4), cell cycle arrest (e.g. baicalein) and combination therapies for antitumor efficacy (a vascular angiogenesis-disrupting agent (e.g. rhES) and 5-FU). |
| Culture Conditions and Handling | |
| Subculturing | Homogenize the cell suspension in the flask by gently pipetting up and down, then take a representative sample to determine the cell density per milliliter. Dilute the suspension with fresh medium to a concentration of 1 x 10^5 cells/mL and aliquot the adjusted suspension into new flasks for continued culture. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | 90% RPMI-1640+10%FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | 60% Basal medium+30% FBS+10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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