Raji Cell Line
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Raji Cell Line
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Raji is the first continuous human cell line of hematopoietic origin and was derived 40 years ago from a patient with Burkitt's lymphoma (BL). Cell lines of BL origin were soon found to harbor the Epstein–Barr virus (EBV), leading to the discovery and isolation of this virus. Subsequent studies revealed specific chromosome translocations, involving the light- or heavy-chain immunoglobulin gene loci on chromosome 2 or 14, and the c-Myc oncogene locus on chromosome 8, in biopsies and cell lines of BL origin. |
| Species | Human |
| Tissue | Lymphoblast |
| Morphology | Lymphoblastoid |
| Disease | Burkitt's lymphoma |
| Age | 11 years |
| Ethnicity | Black |
| Gender | Male |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 2 [Cells contain EBV] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | Cell culture/growth conditions, protein expression |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | 2n = 46, diploid |
| Images |  |
| Sterility | Negative for mycoplasma, bacteria and fungi. |
| Products | Not specified |
| Receptors | Not specified |
| Comments | These cells are EBNA positive. The cells are partially resistant to poliovirus and vesicular stomatitis viruses. This line is tested positive for the presence of Epstein Barr virus (EBV) viral DNA sequences via PCR. |
| Culture Conditions and Handling | |
| Culture Medium | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculturing |
|
| Split Ratio | 1:10 split |
| Medium Renewal | Every 2 to 3 days |
| Freeze Medium | Complete growth medium supplemented with 5% (v/v) DMSO |
| Culture Temperature | 37°C |
| Incubation Condition | carbon dioxide (CO2), 5% |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0115 | GFP Stable Cell Line-Raji | Inquriy |
| CSC-RR0320 | Luc/GFP Reporter Cell Line-Raji | Inquriy |
| CSC-RR0435 | Luciferase Stable Cell Line - Raji | Inquriy |
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