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|Organism||Homo sapiens, human|
|Cell Line Description||Raji is the first continuous human cell line of hematopoietic origin and was derived 40 years ago from a patient with Burkitt's lymphoma (BL). Cell lines of BL origin were soon found to harbor the Epstein–Barr virus (EBV), leading to the discovery and isolation of this virus. Subsequent studies revealed specific chromosome translocations, involving the light- or heavy-chain immunoglobulin gene loci on chromosome 2 or 14, and the c-Myc oncogene locus on chromosome 8, in biopsies and cell lines of BL origin.|
|Biosafety Level||2 [Cells contain EBV]|
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
|Applications||Cell culture/growth conditions, protein expression|
|Shipped in||Dry ice|
|Karyotype||2n = 46, diploid|
|Sterility||Negative for mycoplasma, bacteria and fungi.|
|Comments||These cells are EBNA positive.|
The cells are partially resistant to poliovirus and vesicular stomatitis viruses.
This line is tested positive for the presence of Epstein Barr virus (EBV) viral DNA sequences via PCR.
|Culture Conditions and Handling|
|Culture Medium||RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Split Ratio||1:10 split|
|Medium Renewal||Every 2 to 3 days|
|Freeze Medium||Complete growth medium supplemented with 5% (v/v) DMSO|
|Incubation Condition||carbon dioxide (CO2), 5%|
|CSC-RR0115||GFP Stable Cell Line-Raji||Inquriy|
|CSC-RR0320||Luc/GFP Reporter Cell Line-Raji||Inquriy|
|CSC-RR0435||Luciferase Stable Cell Line - Raji||Inquriy|
The above is only part of a part of cell line products. If you don’t find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.