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Raji Cell Line


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Raji Cell Line

General Information
OrganismHomo sapiens, human
Cell Line DescriptionRaji is the first continuous human cell line of hematopoietic origin and was derived 40 years ago from a patient with Burkitt's lymphoma (BL). Cell lines of BL origin were soon found to harbor the Epstein–Barr virus (EBV), leading to the discovery and isolation of this virus. Subsequent studies revealed specific chromosome translocations, involving the light- or heavy-chain immunoglobulin gene loci on chromosome 2 or 14, and the c-Myc oncogene locus on chromosome 8, in biopsies and cell lines of BL origin.
SpeciesHuman
TissueLymphoblast
MorphologyLymphoblastoid
DiseaseBurkitt's lymphoma
Age11 years
EthnicityBlack
GenderMale
Product FormatFrozen
Growth ModeSuspension
Biosafety Level2 [Cells contain EBV]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ApplicationsCell culture/growth conditions, protein expression
Shipped inDry ice
Storage Temperature−196°C
Characteristics
Karyotype2n = 46, diploid
ImagesRaji Cell Line
SterilityNegative for mycoplasma, bacteria and fungi.
Products  Not specified
ReceptorsNot specified
CommentsThese cells are EBNA positive.
The cells are partially resistant to poliovirus and vesicular stomatitis viruses.
This line is tested positive for the presence of Epstein Barr virus (EBV) viral DNA sequences via PCR.
Culture Conditions and Handling
Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing
  1. If starting from a frozen ampoule the cryoprotectant should be removed.
  2. Add thawed cells to a conical based centrifuge tube, and then slowly add culture medium to the tube.
  3. Take a sample of the cell suspension to count cells.
  4. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes.
  5. Remove medium and re-suspend the cell pellet into fresh medium containing 20% serum at a density of approximately 5 x 105 cells/ml.
  6. Incubate flask at 37°C, check daily and keep the flask in a vertical position until the cells reach the exponential phase of growth.
  7. Once the culture is established the serum concentration can be reduced to 10%. Maintain cultures between 3-9x105 cells/ml.
Split Ratio1:10 split
Medium RenewalEvery 2 to 3 days
Freeze MediumComplete growth medium supplemented with 5% (v/v) DMSO
Culture Temperature37°C
Incubation Conditioncarbon dioxide (CO2), 5%

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