MDA-MB-468 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	MDA-MB-468 is a cell line that was isolated in 1977 from a 51-year-old woman and is commonly used in breast cancer research. The cells are characterized by their epithelial morphology and are known for their high degree of aneuploidy. MDA-MB-468 cells are estrogen receptor negative (ER-) and are commonly used as a model for studying triple-negative breast cancer (TNBC), a subtype of breast cancer that lacks expression of estrogen receptors (ER), progesterone receptors (PR), and HER2/neu. This makes MDA-MB-468 an important tool for studying cancers that do not respond to hormone therapy or HER2-targeted therapies. Genetically, MDA-MB-468 cells exhibit mutations in the TP53 gene, which is common in various forms of cancer and plays an important role in cell cycle regulation and apoptosis. The cell line also exhibits amplification of the epidermal growth factor receptor (EGFR) gene, which facilitates the study of the EGFR signaling pathway and its impact on cancer progression and treatment resistance.
Tissue	Breast; Mammary gland
Disease	Adenocarcinoma
Morphology	Epithelial
Gender	Female
Age	51 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer research 2. Drug discovery and testing 3. Molecular and cellular biology research 4. Biomarker identification 5. Genetic studies 6. Mechanistic studies 7. Immunotherapy research
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	In addition to genetic and phenotypic characteristics, MDA-MB-468 cells are also known for their ability to form xenografts in immunocompromised mice, making them a valuable model for studying tumor growth and metastasis in vivo. The responsiveness of this cell line to various chemotherapeutic agents and targeted therapies has been extensively studied to develop effective TNBC treatment strategies.
Characteristics
Karyotype	Modal number = 64; range = 60 to 67. The cell line is an aneuploid human, presumed female (X, abnormal X), with most chromosome numbers in the subtriploid range.
Antigen Expression	Blood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4 (patient)
Expression Markers	Epidermal growth factor (EGF); transforming growth factor alpha (TGF alpha)
Tumorigenic	Yes, in nude mice.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove old medium from adhered cells and wash with PBS without calcium and magnesium. For T25 flasks, use 3-5 ml PBS and for T75 flasks, use 5-10 ml. 2. Then completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Let the cells incubate at room temperature for 8-10 minutes to allow them to detach. 4. After incubation, gently mix the cells with 10 ml medium to resuspend, then centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells with fresh medium, and transfer them to a new flask already containing fresh medium.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	The ratio of 1:2 to 1:4 is recommended.
Culture Medium	L-15 Medium + 10% FBS
Culture Conditions	Atmosphere: Air, 100%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO0156	Cas9 Stable Cell Line - MDA-MB-468	Inquiry
CSC-RR00693	Luciferase Reporter Cell Line - MDA-MB-468	Inquiry
CSC-RR01040	mScarlet/LUC Reporter Cell Line - MDA-MB-468	Inquiry

* For research use only. Not intended for any clinical use.

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