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circRNA Sequencing Service

Creative Biogene offers comprehensive circRNA sequencing and bioinformatics analysis services supporting basic research and pre-clinical studies. Based on state-of-the-art sequencing platform, we can guarantee superior data quality and accurate sequence assembly, and provide you with the circRNA sequence information with single-base resolution. In addition, our experienced bioinformaticians work closely with you to provide standard and customized circRNA data analysis, including but not limit to, circRNA prediction, identification and annotation, circRNA localization on genome, differential expression analysis of circRNA, GO or KEGG enrichment analysis and circRNA-miRNA/protein interaction network analysis.

As a new type of non-coding RNA, circRNA is expressed endogenously as covalently closed, single-stranded circular molecule. According to its origin, circRNA falls into three categories, Exonic circRNA (ecRNA), Intronic circRNA (ciRNA) and Exon-intron circRNA (ElciRNA). circRNA is abundant and conserved across species, and also exhibits tissue-specific and cell-specific property. Importantly, circRNA is stably expressed in saliva, blood, and exosome, which makes it promising biomarker for diagnosis, prognosis and therapeutic response for cancer patients. Furthermore, several studies have indicated that circRNA exhibited a powerful potential in regulating proliferation, apoptosis, angiogenesis and metastasis, suggesting that circRNA may be important target molecule in cancer. Therefore, the discovery of disease related circRNA may provide a new perspective for the diagnosis and treatment of cancer.

The circRNA sequencing service offered by Creative Biogene produces high quality sequencing data and accurate data analysis to facilitate your research project. All of our services are performed by a group of specially trained and experienced scientists partaking in a streamlined workflow. We are pleased to offer circRNA sequencing and profiling service with the best quality and the most competitive price.

Workflow of circRNA sequencing

Workflow of circRNA sequencing

Sample requirements

  • Total RNA amount ≥ 2 μg
  • OD 260/280 > 1.8, OD 260/230 > 1.0
  • RIN value ≥ 7.0

Recommended sequencing depth

  • 150 bp paired end
  • >40 million reads

Bioinformatics analysis

  • circRNA identification and annotation
  • Statistics of different circRNA types
  • circRNA localization on genome
  • Expression pattern analysis of circRNA
  • Differential circRNA expression analysis
  • Enrichment analysis including GO and KEGG
  • Target miRNA prediction
  • circRNA-miRNA/protein interaction network analysis

Advantages of Creative Biogene

  • The latest NGS technology
  • Experienced staff to assist you with experimental design
  • Fast turnaround time
  • Comprehensive bioinformatics analysis

For further information about our circRNA sequencing service, please feel free to contact us at or 1-631-626-9181.

Reference:

  1. Zhang, M. (2018). Circular RNAs: a new frontier for cancer diagnosis and therapy. Journal of Hematology & Oncology, 11(1), 21.

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