CRISPR/Cas9 is a revolutionary technology in the field of gene engineering. It has expedited the course of biopharmaceutical...
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CRISPR/Cas9 is a revolutionary technology in the field of gene engineering. It has expedited the course of biopharmaceutical research and drug discovery, by providing scientists with a versatile tool to knockout any gene, in any cell and without introducing foreign DNA. The benefits of CRISPR/Cas9 over previous forms of gene editing, such as TALENs and zinc finger nuclease (ZFN), are that it is much simpler to implement and has higher efficiency at performing bi-allelic gene modifications.
Creative Biogene is experienced to provide a full CRISPR/Cas9-based single gene knockout service using any mammalian cell line and targeting any gene. Our scientists are experts at performing gene knockout with CRISPR/Cas9, from designing gRNA constructs to transfection and single clone generation of a wide range of cells, including difficult-to-transfect and tumor cell lines.
Our services include:
Host cell characterization
gRNA design and KO vector construction
Single cell screening
Single Gene Knock Out Cell Line Generation Service Case
Knock out the Fut8 gene in CHO-K1 cells
1. Genomic sequencing results of Fut8 gene KO cell lines
2. qPCR analysis of Fut8 gene KO cell lines