Monkeypox Virus Related Cell Lines

Monkeypox virus belongs to the Orthopoxvirus genus of the Poxviridae family. It is an enveloped double-stranded DNA virus with a genome of about 200 kb encoding about 200 proteins. Some of these proteins are involved in the key steps of viral replication to ensure the smooth progress of viral replication, while others are responsible for antagonizing the host immune response and achieving immune escape. These proteins are essential for viral replication and can be used as potential drug targets.

Schematic diagram of localization of proteins in monkeypox virus.
Adapted from Principles of Molecular Virology, 6th Edition (p. 46), by Alan J. Cann, 2016, UK: Elsevier.

A35R

A35R is an envelope glycoprotein on the EV surface of monkeypox virus and plays an important role in the cell-to-cell transmission of virions.

L1R

L1R is homologous to the J1R protein of vaccinia virus, which is an essential membrane protein for virus growth and plaque formation, and plays a role in the formation of immature virus particles.

A29L

A29L is the surface membrane protein of monkeypox virus IMV, which is homologous to the A27L protein of vaccinia virus. A27L can bind to heparin or heparan sulfate on the cell surface to promote cell fusion.

M1R

M1R is the surface membrane protein of monkeypox virus IMV, which is homologous to the L1R protein of vaccinia virus and plays an important role in the process of virus entry and maturation.

E8L

E8L is the monkeypox virus cell surface-binding protein, which can combine with chondroitin sulfate on the cell surface, allowing the virion to attach to the target cell.

B6R

B6R is located on the monkeypox virus EEV membrane, which is homologous to the B5R protein of vaccinia virus, and plays an important role in the encapsulation of IMV in the intracellular membrane, the formation of EEV, the normal plaque size, and the virulence of the virus.

B21R

Studies have shown that the 1780-1810 and 1840-1870 peptide segments of B21R protein have good specificity and sensitivity in serological detection.

Creative Biogene has developed Monkeypox Virus reporter cell lines that will assist monkeypox-related research and satisfy antibody screening.

Listed below are the monkeypox proteins most frequently used for antibody development:

References:

Dubois ME, Hammarlund E, Slifka MK. Optimization of peptide-based ELISA for serological diagnostics: a retrospective study of human monkeypox infection. Vector Borne Zoonotic Dis. 2012;12(5):400-409.
Hammarlund E, Lewis MW, Carter SV, et al. Multiple diagnostic techniques identify previously vaccinated individuals with protective immunity against monkeypox. Nat Med. 2005;11(9):1005-1011.
Roumillat LF, Patton JL, Davis ML. Monoclonal antibodies to a monkeypox virus polypeptide determinant. J Virol. 1984;52(1):290-292.

Cat.No.	Product Name	Price
CSC-RO1096	Monkeypox Virus A35R-EGFP Stable Cell Line-HEK293	Inquiry
CSC-RO1097	Monkeypox Virus E8L-EGFP Stable Cell Line-HEK293	Inquiry
CSC-RO1098	Monkeypox Virus A28L-EGFP Stable Cell Line-HEK293	Inquiry
CSC-RO1099	Monkeypox Virus A29L-EGFP Stable Cell Line-HEK293	Inquiry
CSC-RO1100	Monkeypox Virus A30L-EGFP Stable Cell Line-HEK293	Inquiry
CSC-RO1101	Monkeypox Virus B21R-EGFP Stable Cell Line-HEK293	Inquiry
CSC-RO1102	Monkeypox Virus B6R-EGFP Stable Cell Line-HEK293	Inquiry
CSC-RO1103	Monkeypox Virus L1R-EGFP Stable Cell Line-HEK293	Inquiry
CSC-RO1104	Monkeypox Virus H3L-EGFP Stable Cell Line-HEK293	Inquiry
CSC-RO1105	Monkeypox Virus I1L-EGFP Stable Cell Line-HEK293	Inquiry
CSC-RO1106	Monkeypox Virus M1R-EGFP Stable Cell Line-HEK293	Inquiry

* For research use only. Not intended for any clinical use.

Quick Inquiry