Vectors
Product DetailsApplicationCase StudyFAQ
Product Details
Creative Biogene provides a diverse selection of vectors, including pDest22, pG-KJE8, pCMV-BD, and pTALEN v2, tailored for various molecular biology and genetics applications. Our vectors, equipped with customizable features, empower researchers to accelerate scientific discoveries in gene cloning, expression analysis, protein interaction studies, and genome editing. Choose Creative Biogene for high-quality vectors to meet your research needs.Key Features of Our General Vectors.
- Diversity and Customization: Creative Biogene offers a variety of meticulously designed vector types tailored to specific experimental needs, with customizable features for versatility across different settings.
- Stability and Reliability: Our vectors undergo rigorous quality control and validation processes, ensuring stability and reliability for consistent, high-quality results.
- Wide Range of Applications: Our vectors are suitable for gene cloning, expression analysis, protein-protein interaction studies, genome editing, and other molecular biology applications, showcasing their versatility and utility.
- Quality Service and Innovation: We provide professional technical support, and high-quality customer service, and continually innovate by releasing new vector products and enhancing existing ones to meet evolving customer demands and drive industry advancements.
A versatile tool for inserting, replicating, and amplifying DNA fragments, featuring multiple restriction enzyme recognition sites and selectable markers.
Facilitates efficient expression of exogenous genes within host cells, containing promoters, regulatory sequences, and selectable markers.
Enables stable replication in bacteria and rapid separation and screening of DNA fragments, useful for applications such as antibody library construction and protein-protein interaction screening.
Flexible molecular cloning tools for DNA fragment insertion, replication, and transfer in various molecular biology experiments.
Browse All Vectors
Application
Vectors play a pivotal role in molecular biology and genetic engineering by providing versatile tools for various research applications. These vectors serve as carriers for the insertion, manipulation, and expression of DNA fragments in a wide range of experimental settings. With diverse features and functionalities, vectors cater to the specific needs of researchers across different fields. They are instrumental in gene cloning, protein expression, gene editing, signal pathway analysis, and functional genomics research. These vectors enable scientists to investigate gene function, regulatory mechanisms, protein-protein interactions, and cellular processes. Creative Biogene can assist with the following research:
- Gene expression and purification experiments: Using vectors such as pQE, pRSET, pGEX, and pMAL for protein expression and purification to obtain high-purity target proteins.
- Protein-protein interaction studies: Conducting protein interaction experiments using vectors containing tagged or fusion proteins (such as pGEX and pMAL) to explore protein-protein interactions.
- Molecular cloning and genome library construction: Utilizing vectors with appropriate restriction enzyme cleavage sites for gene cloning and library construction, for molecular biology research.
- Transgenic and gene editing experiments: Employing vectors such as pUC19 and pGAPZ for gene transfection, transformation, or editing, to achieve transgenic animal model construction or gene editing technology application.
- Drug screening and functional genomics research: Conducting drug screening experiments or CRISPR/Cas9-mediated genome editing experiments using vectors, to advance drug development and functional genomics research.
Case Study
Echocardiographic parameters of infarcted AAV9-shXPO1 and AAV9-scramble rats measured before surgery and injection (stage 0), before injection (stage I), and 2 (stage II) and 5 months (stage III) after vector injection.
Paper
2020
Journal of Cardiovascular Translational Research |
DOI
Background
This study investigates whether AAV9-mediated XPO1 gene silencing can improve the cardiac function of rats with myocardial infarction. The authors induced chronic myocardial infarction in Sprague-Dawley rats by ligating the coronary artery, and then intravenously injected AAV9-shXPO1 or control AAV9-scramble. The results showed that AAV9-shXPO1 could reduce the expression of EXP-1 in the heart, partially restore the left ventricular short-axis shortening rate and maintain the left ventricular size, while reducing collagen fibers and fibrosis, and did not significantly change the levels of IL-6 or TNFR1. The study demonstrated that XPO1 silencing can alleviate cardiac dysfunction and remodeling after myocardial infarction, providing a new strategy for gene therapy of ischemic cardiomyopathy.
Key Findings
In the chronic myocardial infarction rat model, intravenous injection of AAV9-shXPO1 specifically reduced the level of EXP-1 in the heart, while having no significant effects on skeletal muscle, liver, and brain. After 5 months of treatment, the left ventricular short-axis shortening rate in the AAV9-shXPO1 group recovered from 16.8 ± 2.8% to 24.6 ± 4.1%, and the left ventricular systolic and diastolic end diameters also improved. However, there was no recovery in the AAV9-scramble group. Histology showed that the collagen fibers and fibrotic area in the AAV9-shXPO1 group decreased. There were no significant differences in plasma IL-6 and TNFR1 between the two groups, suggesting no obvious systemic inflammatory side effects. The results indicate that XPO1 silencing can alleviate ventricular remodeling and cardiac dysfunction after myocardial infarction, and the AAV9 vector has cardiac preference and long-term efficacy.
Contribution
Creative Biogene is responsible for preparing the AAV9-shXPO1 and the control AAV9-scramble vectors used in this study. The vectors provided by them achieved the specific downregulation of EXP-1 in the heart muscle, enabling the authors to verify the therapeutic effect of XPO1 silencing on the cardiac function and ventricular remodeling of rats with myocardial infarction. They are the key gene delivery tool and material support provider for this study.
Antiviral effects of PC786. (A) Chemical structure of PC786.
Paper
2017
Antimicrobial Agents and Chemotherapy |
DOI
Background
PC786 is an inhaled small-molecule nonnucleoside inhibitor targeting the RSV L protein polymerase. This study reports preclinical profiling, showing potent, selective antiviral activity against RSV-A and RSV-B laboratory and clinical isolates in HEp-2 cells without detectable cytotoxicity. PC786 inhibits viral replication, retains activity at high MOI and after established infection, and demonstrates persistent effects in human bronchial epithelial cells. Once-daily intranasal dosing reduces viral load to undetectable levels in RSV-infected mice and cotton rats. A dominant L protein mutation (Y1631H) was identified, and PC786 inhibits RSV RNA-dependent RNA polymerase in cell-free and minigenome assays, supporting inhaled topical therapy.
Key Findings
PC786 showed potent, selective anti-RSV activity in HEp-2 CPE assays, with IC50 values <0.09–0.71 nM for RSV-A and 1.3–50.6 nM for RSV-B clinical and laboratory isolates, without cytotoxicity up to 14 μM. PCR confirmed inhibition of viral replication. Activity was largely unaffected by MOI and retained when treatment began up to 48 h after infection, unlike the F inhibitor GS-5806. Persistent antiviral effects were observed in human bronchial epithelial cells and air-liquid interface cultures. Once-daily intranasal or intratracheal PC786 reduced lung viral loads to undetectable levels in RSV-infected mice and cotton rats and decreased RANTES/IP-10 transcripts. Escape mutant analysis identified a dominant L protein mutation (Y1631H). PC786 inhibited RSV RdRp activity in cell-free enzyme (IC50 2.1 nM) and minigenome (IC50 0.5 nM) assays, with greater potency than AZ-27, BI compound D, ribavirin, and ALS-8112. These data support PC786 as a novel inhaled anti-RSV therapeutic.
Contribution
Creative Biogene provided key plasmid reagents, including the RSV trailer/promoter-conjugated luciferase minigenome plasmid, wild-type M protein expression vector, and mutant L protein expression vector with the Y1631H mutation. These reagents enabled mechanism-of-action studies, minigenome assays, phenotypic validation of PC786 resistance, and mapping of the L protein target.
Case Study 1
PDCoV poses a recent and growing menace to the worldwide pig farming sector. Researchers systematically investigated the role of trypsin in Porcine delta coronavirus (PDCoV) replication, focusing on cell entry, cell-to-cell membrane fusion, and virus release. Using pseudovirus entry assays, they found PDCoV entry is not trypsin-dependent, unlike the Porcine epidemic diarrhea virus (PEDV). Trypsin was shown to promote PDCoV replication by enhancing cell-to-cell membrane fusion, elucidating distinct spreading patterns, and its role in different virus transmission types. These findings highlight trypsin's significance in PDCoV replication and its potential for enhancing virus production efficiency and developing antiviral treatments, crucial for mitigating its threat to the global porcine industry.
Figure 1. PDCoV spike-mediated cell-to-cell membrane fusion was studied by researchers using pACT-MyoD. HEK293-APN cells were co-transfected with pBind-Id and PACT-MyoD, then mixed with other HEK293-APN cells expressing PDCoV spike and PGL5-Luc. Trypsin was employed to modulate fusion efficiency, with luciferase activity serving as an indicator. (Yang YL, et al., 2020)
Case Study 2
Researchers have developed an episome-based assay utilizing pECFP-1 to investigate RAG-mediated transposition in human embryonic kidney cells (HEK 293T). This assay aims to elucidate whether RAG proteins function as transposases in vertebrate cells and assess the potential genomic instability caused by RAG-mediated DNA cleavage products. Results show low-frequency transposition events with characteristic target site duplications, accompanied by deletions or complex rearrangements distinct from transposition. Assays targeting transposition into the human genome revealed chromosome deletion and translocation events involving RAG-mobilized signal end fragments. These findings shed light on the mechanisms and consequences of RAG-mediated DNA cleavage, highlighting the importance of understanding RAG protein behavior in vertebrate cells.
Figure 2. In an episomal assay for RAG-mediated transposition, pECFP-1 was used as the target plasmid by researchers. By introducing a tetracycline resistance gene into the target plasmid, successful integration events in transfected 293T cells were assessed, enabling survival on agar plates containing KTS. (Chatterji M, et al., 2006)
FAQ
Q: What are the specific features of Creative Biogene's vector products?
A: Creative Biogene's vector products include a variety of commonly used plasmid and viral vectors, with rich functional modules such as selection markers, promoters, and terminators, suitable for a wide range of gene cloning, expression, and editing experiments.
Q: How do I choose the vector product that suits my experiment?
A: Our vector products cover various vector types and functional features. You can choose a vector with appropriate restriction enzyme sites, promoters, or selection markers according to your experimental needs to ensure it fits your experimental design.
Q: What is the quality of Creative Biogene's vector products?
A: Creative Biogene is committed to providing high-quality vector products. All products undergo strict quality control and validation processes to ensure stability, purity, and reliability.
Q: Is it possible to customize specific vector products?
A: Yes, Creative Biogene can customize specific vector products according to customer requirements, including the design of specific restriction enzyme sites, promoters, selection markers, and other functional modules.
Q: How can I obtain more information about vector products?
A: You can browse our product catalog on our website to obtain detailed information, or you can directly contact our customer service team, who will be happy to provide support and assistance.
* For research use only. Not intended for any clinical use.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
