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Creative Biogene provides a diverse selection of vectors, including pDest22, pG-KJE8, pCMV-BD, and pTALEN v2, tailored for various molecular biology and genetics applications. Our vectors, equipped with customizable features, empower researchers to accelerate scientific discoveries in gene cloning, expression analysis, protein interaction studies, and genome editing. Choose Creative Biogene for high-quality vectors to meet your research needs.Key Features of Our General Vectors.
A versatile tool for inserting, replicating, and amplifying DNA fragments, featuring multiple restriction enzyme recognition sites and selectable markers.
Facilitates efficient expression of exogenous genes within host cells, containing promoters, regulatory sequences, and selectable markers.
Enables stable replication in bacteria and rapid separation and screening of DNA fragments, useful for applications such as antibody library construction and protein-protein interaction screening.
Flexible molecular cloning tools for DNA fragment insertion, replication, and transfer in various molecular biology experiments.
Vectors play a pivotal role in molecular biology and genetic engineering by providing versatile tools for various research applications. These vectors serve as carriers for the insertion, manipulation, and expression of DNA fragments in a wide range of experimental settings. With diverse features and functionalities, vectors cater to the specific needs of researchers across different fields. They are instrumental in gene cloning, protein expression, gene editing, signal pathway analysis, and functional genomics research. These vectors enable scientists to investigate gene function, regulatory mechanisms, protein-protein interactions, and cellular processes. Creative Biogene can assist with the following research:
Case Study 1
PDCoV poses a recent and growing menace to the worldwide pig farming sector. Researchers systematically investigated the role of trypsin in Porcine delta coronavirus (PDCoV) replication, focusing on cell entry, cell-to-cell membrane fusion, and virus release. Using pseudovirus entry assays, they found PDCoV entry is not trypsin-dependent, unlike the Porcine epidemic diarrhea virus (PEDV). Trypsin was shown to promote PDCoV replication by enhancing cell-to-cell membrane fusion, elucidating distinct spreading patterns, and its role in different virus transmission types. These findings highlight trypsin's significance in PDCoV replication and its potential for enhancing virus production efficiency and developing antiviral treatments, crucial for mitigating its threat to the global porcine industry.
Figure 1. PDCoV spike-mediated cell-to-cell membrane fusion was studied by researchers using pACT-MyoD. HEK293-APN cells were co-transfected with pBind-Id and PACT-MyoD, then mixed with other HEK293-APN cells expressing PDCoV spike and PGL5-Luc. Trypsin was employed to modulate fusion efficiency, with luciferase activity serving as an indicator. (Yang YL, et al., 2020)
Case Study 2
Researchers have developed an episome-based assay utilizing pECFP-1 to investigate RAG-mediated transposition in human embryonic kidney cells (HEK 293T). This assay aims to elucidate whether RAG proteins function as transposases in vertebrate cells and assess the potential genomic instability caused by RAG-mediated DNA cleavage products. Results show low-frequency transposition events with characteristic target site duplications, accompanied by deletions or complex rearrangements distinct from transposition. Assays targeting transposition into the human genome revealed chromosome deletion and translocation events involving RAG-mobilized signal end fragments. These findings shed light on the mechanisms and consequences of RAG-mediated DNA cleavage, highlighting the importance of understanding RAG protein behavior in vertebrate cells.
Figure 2. In an episomal assay for RAG-mediated transposition, pECFP-1 was used as the target plasmid by researchers. By introducing a tetracycline resistance gene into the target plasmid, successful integration events in transfected 293T cells were assessed, enabling survival on agar plates containing KTS. (Chatterji M, et al., 2006)
Q: What are the specific features of Creative Biogene's vector products?
A: Creative Biogene's vector products include a variety of commonly used plasmid and viral vectors, with rich functional modules such as selection markers, promoters, and terminators, suitable for a wide range of gene cloning, expression, and editing experiments.
Q: How do I choose the vector product that suits my experiment?
A: Our vector products cover various vector types and functional features. You can choose a vector with appropriate restriction enzyme sites, promoters, or selection markers according to your experimental needs to ensure it fits your experimental design.
Q: What is the quality of Creative Biogene's vector products?
A: Creative Biogene is committed to providing high-quality vector products. All products undergo strict quality control and validation processes to ensure stability, purity, and reliability.
Q: Is it possible to customize specific vector products?
A: Yes, Creative Biogene can customize specific vector products according to customer requirements, including the design of specific restriction enzyme sites, promoters, selection markers, and other functional modules.
Q: How can I obtain more information about vector products?
A: You can browse our product catalog on our website to obtain detailed information, or you can directly contact our customer service team, who will be happy to provide support and assistance.
| Cat.No. | Product Name | Price |
|---|---|---|
| OVT2505 | pDest22 | Inquiry |
| OVT2506 | pDest32 | Inquiry |
| OVT2507 | pG-KJE8 | Inquiry |
| OVT2508 | pGro7 | Inquiry |
| OVT2509 | pKJE7 | Inquiry |
| OVT2510 | pG-Tf2 | Inquiry |
| OVT2511 | pTf16 | Inquiry |
| OVT2512 | pACT | Inquiry |
| OVT2513 | pBIND | Inquiry |
| OVT2514 | pACT-MyoD | Inquiry |
| OVT2515 | pG5 luc | Inquiry |
| OVT2516 | pCMV-BD | Inquiry |
| OVT2517 | pCMV-AD | Inquiry |
| OVT2518 | pBD-p53 | Inquiry |
| OVT2519 | pBD-NF?κB | Inquiry |
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