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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Caki-2 is a human renal cell line established in 1971, derived from a primary clear cell renal cell carcinoma (ccRCC) in a 69-year-old Caucasian male. This cell line exhibits an epithelial morphology and is characterized by adherent growth. Although Caki-2 is frequently compared with the Caki-1 cell line (which was derived from a metastatic lesion), Caki-2 actually originates from a primary tumor and is distinguished by a slower growth rate and unique genetic characteristics. In the field of urologic oncology, Caki-2 serves as a critical model, frequently utilized to investigate the mechanisms underlying the pathogenesis and progression of renal malignancies, cellular metabolic processes, and the efficacy of targeted therapies—such as tyrosine kinase inhibitors (TKIs). |
| Tissue | Kidney |
| Disease | Clear Cell Renal Cell Carcinoma (ccRCC) |
| Morphology | Epithelial |
| Gender | Male |
| Age | 69 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Renal cell carcinoma (RCC) pathogenesis and progression research 2. Study of kidney-specific gene expression and regulation 3. Drug screening and cytotoxicity assays for renal cancer 4. Investigation of tumor angiogenesis and VHL signaling pathways 5. Development of human tumor xenograft models in nude mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Karyotype | Aneuploid; modal number in the triploid range; multiple chromosomal rearrangements |
| Genetic Profile | VHL wild-type (unlike many other ccRCC lines); TP53 wild-type; PTEN wild-type |
| Expression Markers | Positive for specific kidney isoenzymes; G6PD type B |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with PBS (without Ca2+/Mg2+) to remove all traces of serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin - 0.53 mM EDTA solution and observe under an inverted microscope until the cell layer is dispersed (usually 5 to 15 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to obtain a single-cell suspension. 5. Aliquot cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00743 | Luciferase Reporter Cell Line - Caki-2 | Inquiry |
| CSC-RR00744 | GFP Reporter Cell Line - Caki-2 | Inquiry |
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