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Genome-editing tools efficiently introduce mutations or modifications into diverse cell genomes. Recombinases, such as Cas9, Cre, and optimized FLPo, induce site-specific double-strand breaks. Isogenic models created using these tools facilitate disease study and human gene therapy. Creative Biogene offers ready-to-use recombinase lentiviral particles expressed under various promoters with markers. Our prepackaged lentiviruses undergo rigorous Q&C for high-quality delivery. Please let us know your requirements.
Key Features of Our Recombinase Lentiviral Particles
Recombinase Lentiviral Particles Product List
Site-specific recombinases like Cre enable precise genomic manipulation through the recognition of short target sequences like loxP sites. Lentiviral delivery allows robust expression in cells containing pre-engineered loxP sites flanking genomic regions of interest. This mediates efficient sequence excision, inversion, or translocation between the loxP sites. The stably integrating lentiviral system provides an advantage over adenoviruses. Perhaps your research work requires tools in the following directions:
Case Study 1
Gliomas constitute the predominant and diverse category of primary brain tumors. Researchers utilized the Cre/loxP recombination system to advance animal models investigating the intricate landscape of malignant gliomas. The introduction of GFAP-FLPo mice expressing FLPo recombinase in GFAP-positive cells, coupled with lentivirus-based in vivo delivery of cancer genes through FLP/FRT-mediated recombination, enabled the initiation of gliomas. Leveraging the Cre-mediated multi-fluorescent protein expressing system, the GFAP-FLPo mouse model facilitates the analysis of diverse gliomagenesis stages. This novel mouse model expands our capacity to dissect developmental processes in gliomagenesis, offering new avenues for therapeutic exploration.
Figure 1. The FLPo recombinase was inserted into the first exon of the GFAP cassette. After verifying FLPo recombination activity in vitro, GFAP-FLPo founder lines were generated via pronuclear injection of the linearized GFAP-FLPo construct. (Hara T, et al., 2019)
Case Study 2
Techniques utilizing site-specific recombinases are extensively employed to investigate gene activities both in vivo andin vitro. Researchers employed lentiviral vectors to address the limitations of current gene tracing methods based on site-specific recombinases. These vectors enable the expression of constitutively active or inducible variants of recombinases under lineage-specific or ubiquitous promoters. The study aims to advance the development of schemes allowing autonomous initiation of lineage tracing at a chosen time point. Notably, the investigation focuses on overcoming limitations in tracing the key germline gatekeeper gene Oct4 beyond the peri-implantation epiblast stage, highlighting the relevance of establishing genetic tools for such purposes.
Figure 2. O4S ESC-derived neural cells were sensitized and reprogrammed using lentivirally delivered OKSM factors. tdTomato expression was detected by day 6–7 post-infection. (Kuzmin AA, et al., 2019)
Q: Which types of recombinase enzymes are commonly delivered by these lentiviral particles, and what are their roles?
A: Commonly delivered recombinase enzymes include Cre and Flp. Cre mediates recombination between specific DNA recognition sites (loxP), while Flp acts on FRT (Flp recombinase target) sites, allowing site-specific DNA modifications.
Q: In what experimental scenarios are Recombinase Lentiviral Particles particularly useful?
A: These particles are useful in experiments requiring precise genetic modifications, such as generating conditional knockout or knock-in models, studying gene function, and manipulating DNA sequences for controlled expression.
Q: How do researchers ensure the efficiency and specificity of recombinase-mediated recombination using lentiviral delivery?
A: Researchers optimize lentiviral titers, transduction conditions, and use recombinase enzymes with high specificity to achieve efficient and specific DNA recombination, minimizing off-target effects.
Q: How do Recombinase Lentiviral Particles contribute to advancements in gene therapy research and applications?
A: These particles contribute by facilitating controlled genetic modifications, which is crucial for developing safer and more effective gene therapies with precise control over therapeutic gene expression.
| Cat.No. | Product Name | Price |
|---|---|---|
| LV00948Z | Synapsin-Cre Lentivirus | Inquiry |
| LV00953Z | CMV-SpCas9 Lentivirus | Inquiry |
| LV00954Z | EF1α-SpCas9 Lentivirus | Inquiry |
| LV00960Z | CMV-Cre-Puro Lentivirus | Inquiry |
| LV00961Z | CMV-iCre-Puro Lentivirus | Inquiry |
| LV00965Z | CMV-Cre-GFP-Puro Lentivirus | Inquiry |
| LV00966Z | CMV-Cre-mCherry-Puro Lentivirus | Inquiry |
| LV00967Z | EF1α-Cre-Puro Lentivirus | Inquiry |
| LV00968Z | EF1α-iCre-Puro Lentivirus | Inquiry |
| LV00969Z | EF1α-Cre-GFP-Puro Lentivirus | Inquiry |
| LV00970Z | EF1α-Cre-mCherry-Puro Lentivirus | Inquiry |
| LV00971Z | CAG-Cre Lentivirus | Inquiry |
| LV00972Z | CAG-Cre-GFP Lentivirus | Inquiry |
| LV00973Z | CAG-Cre-mCherry Lentivirus | Inquiry |
| LV00980Z | Synapsin-iCre Lentivirus | Inquiry |
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