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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SW48 cell line was established from a grade IV ulcerating tumor encircling the bowel of an 82-year-old Caucasian female. The SW48 cell line is commonly used in cancer research and drug development studies due to its ability to grow rapidly in culture and its genetic similarities to human colon cancer. |
| Tissue | Colon |
| Disease | Human colorectal adenocarcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 82 years |
| Product Format | Frozen |
| Growth Mode | Adherent or attaching to the culture flask |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 3D cell culture Cancer biology study Drug screening Drug resistance study |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | SW48 cells have higher expression of invasion-mediating genes, especially MMP2, MMP9, and angiogenic factors such as vascular endothelial growth factor (VEGF). Furthermore, this cell line showed the highest invasion and migration activity in Transwell migration and invasion assays, making it a suitable cell model for in vitro studies. |
| Characteristics | |
| Tumorigenic | Yes, tumors form in nude mice. |
| Mycoplasma Test | Negative |
| Bacteria Test | Negative |
| Yeast Test | Negative |
| Antigen Expression | HLA A32, A33, B27, B35; blood type AB; Rh+ |
| Cytogenetic Information | 2n = 46 |
| Culture Conditions and Handling | |
| Subculturing | 1. Culture never becomes 100% confluent. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. To remove the trypsin-EDTA solution, transfer the cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | 1 to 2 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:6 is recommended. |
| Culture Medium | Leibovitz's L-15 Medium + 10% Fetal Bovine Serum (FBS). |
| Culture Conditions | Air: 100%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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