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miRNA Service

Custom miRNA Microarray Service
Library-based Functional miRNA Screening Service
Microarray-based miRNA Expression Profiling Service
MicroRNA Agomir/ Antagomir Synthesis Service
MicroRNA Sponge Service
HighlightsServiceCase StudySupportFAQ

MicroRNAs (miRNAs) are small noncoding RNA molecules, typically around 20-25 nucleotides in length, that play critical roles in regulating gene expression. These tiny molecules exert their regulatory effects by binding to the 3' untranslated regions (3'UTRs) of target mRNAs, either causing their degradation or inhibiting their translation. As a result, miRNAs are central to various biological processes such as cell differentiation, proliferation, and apoptosis. Dysregulation of miRNAs has been implicated in numerous diseases, including cancer, cardiovascular diseases, and neurodegenerative disorders.

Creative Biogene offers a comprehensive suite of miRNA services designed to accelerate your research and contribute to groundbreaking discoveries. With our advanced technologies, cutting-edge equipment, and highly experienced experts, we provide tailored miRNA services that meet your needs in miRNA profiling, screening, synthesis, and functional studies. Our services aim to support researchers in understanding the role of miRNAs in cellular processes and disease mechanisms.

Service Highlights

1 Expertise & Experience

Over 10 years of experience in miRNA research and applications. Completed >50 miRNA projects with a strong publication track record (Bioengineered, JECC).

2 Comprehensive & Scalable Assays

Assays covering 2,588 known human miRNAs. Scalable from individual miRNAs to genome-wide studies.

3 Reliable Delivery & Cost Efficiency

>95% on-time delivery rate. Competitive pricing for all project sizes. Bulk order discounts.

miRNA Services

Custom miRNA Microarray Service

Utilizing the latest in microarray technology, we offer flexible, high-throughput solutions for profiling miRNA expression in a wide range of sample types.

  • Maximum flexibility in microarray design and content
  • High coverage, uniformity, specificity, and sensitivity

Library-based Functional miRNA Screening Service

To screen large libraries of miRNAs to identify candidates involved in regulating specific cellular processes, such as cell proliferation, differentiation, and apoptosis.

  • Screening using miRNA mimic or inhibitor libraries
  • Identification of novel miRNA functions and pathways
  • High-throughput capabilities with advanced screening technologies

Microarray-based miRNA Expression Profiling Service

Creative Biogene provides Microarray-based miRNA Expression Profiling to identify and quantify miRNA expression across multiple conditions.

  • High-quality expression profiling of miRNAs
  • Comprehensive data analysis including differential expression
  • Suitable for a wide range of sample types

MicroRNA Agomir/ Antagomir Synthesis Service

Agomirs are miRNA mimics designed to upregulate target gene expression, while Antagomirs are inhibitors that block miRNA activity.

  • Covers all human, mouse, and rat miRNAs listed in miRBase
  • Enhanced stability and easy transfection

MicroRNA Sponge Service

The MicroRNA Sponge Service is designed for long-term loss-of-function studies of miRNAs. Our service offers a valuable tool for functional studies both in vitro and in vivo.

  • Stable expression of multiple miRNAs using plasmids or lentiviruses
  • Customizable vectors with different promoters and reporters
  • Long-term expression for sustained knockdown

Client Case Studies & Research Outcomes

Case Study 1

Gastric cancer (GC) is one of the most prevalent cancers worldwide, with a high mortality rate. Advances in understanding its molecular mechanisms are essential for developing targeted therapies. Recent studies have highlighted the LINC00240/miR-338-5p/METTL3 axis as a key regulator of GC progression, offering potential targets for intervention.

The researchers explored the role of this axis in GC cell aggressiveness. They found that LINC00240 was upregulated in GC tissues and associated with poor prognosis. Using miR-338-5p mimic and inhibitor products from Creative Biogene, they demonstrated that miR-338-5p acts as a downstream target negatively regulated by LINC00240 and suppresses METTL3 expression. Overexpression of METTL3 or inhibition of miR-338-5p reversed the anti-proliferative and pro-apoptotic effects of LINC00240 knockdown.

Figure 1 demonstrates the bioinformatics-predicted binding site between LINC00240 and miR-338-5p, and validates this interaction through qRT-PCR, dual luciferase reporter assay. (doi: 10.1080/21655979.2021)Figure 1. The researchers investigated the interaction between LINC00240 and miR-338-5p using bioinformatics, qRT-PCR, and dual luciferase reporter assays. They confirmed the binding site and functional relationship, demonstrating the negative regulation of miR-338-5p by LINC00240 in gastric cancer tissues.(Wang G, et al., 2021)

Case Study 2

Myotonic dystrophy type 1 (DM1) is the most common adult hereditary myopathy, characterized by progressive skeletal muscle degeneration leading to severe disability. Advances in understanding DM1’s molecular pathology have enabled the development of novel therapeutic approaches. The researchers aimed to overcome limitations of existing preclinical models by creating the first 3D in vitro model of DM1 skeletal muscle. Using patient-derived fibroblasts transdifferentiated into myoblasts, they engineered 3D microtissues with gelatin methacryloyl-based hydrogels. These biomimetic cultures promoted myoblast alignment and differentiation into myotubes, effectively recapitulating molecular and structural disease hallmarks seen in patient biopsies. Notably, 3D micropatterning significantly improved DM1 myotube differentiation and revealed phenotypic markers like reduced myotube thickness, which serve as metrics for drug testing. The researchers administered antagomiR-23b oligonucleotides, procured from Creative Biogene to DM1 microtissues.

Figure 2 illustrates the therapeutic effects of antagomiR-23b in a 3D in vitro model of Myotonic Dystrophy Type 1 (DM1), showing its impact on miR-23b expression, MBNL1/MBNL2 levels, alternative splicing events, myotube morphology, and diameter through comprehensive molecular and cellular analyses. (doi:10.1088/1758-5090/abf6ae)Figure 2. The researchers evaluated the therapeutic potential of antagomiR-23b on a 3D DM1 human skeletal muscle model by analyzing miR-23b expression, MBNL1/2 levels, splicing events, and myotube structure, demonstrating its efficacy in restoring molecular and structural hallmarks of DM1. (Fernández-Garibay X, et al., 2021)

Case Study 3

The researchers aimed to investigate the influence of neutrophil-derived microvesicles on arterial pathophysiology, particularly focusing on their delivery of miR-155, a microRNA known to enhance NF-κB activation and inflammatory gene expression. Using cultured endothelial cells under disturbed flow conditions, the researchers demonstrated that neutrophil microvesicles significantly increase miR-155 levels and inflammatory responses. Furthermore, in a murine model, these microvesicles were found to accumulate in disease-prone arterial regions, exacerbating atherosclerotic plaque formation and macrophage content.

The researchers utilized Creative Biogene’s miRNA services by transfecting endothelial cells with miR-155 antagomir or a scrambled control antagomir. This approach effectively demonstrated the critical role of miR-155 in promoting vascular inflammation and atherogenesis. The antagomirs, transfected using Lipofectamine, suppressed miR-155 activity, providing insights into potential therapeutic strategies targeting miRNA-mediated inflammation.

Figure 3 comprehensively investigates miRNA content in neutrophil-derived membrane vesicles (NMVs). (doi: 10.1038/s41467-019-14043-y) Figure 3. The researchers employed RT-qPCR to quantify miRNA content in neutrophil microvesicles (NMVs) isolated from human and mouse plasma, both before and after high-fat diet exposure. They studied the effects of miR-155 on gene expression in HCAECs and evaluated the impact of miR-155 antagomir treatment on inflammatory gene regulation. (Gomez I, et al. 2020)

Service Support

Our services are supported by a team of experts in the field of miRNA research who are dedicated to helping you achieve your research goals. From initial consultation to the final data analysis, we provide comprehensive support throughout the project lifecycle. Whether you require assistance with experimental design, technical advice, or data interpretation, Creative Biogene is here to guide you every step of the way.

We also offer flexible collaboration options, ensuring that our services can be tailored to fit the specific requirements of your research projects. For special requests or additional services, our team is always ready to discuss custom solutions that meet your needs.

Creative Biogene’s miRNA services provide the tools, expertise, and support you need to advance your research. Whether you are investigating miRNA expression, screening for functional roles, or synthesizing customized miRNAs, we are committed to delivering high-quality, reliable results that accelerate your scientific discoveries.

FAQ

Q: Does the service include data analysis?

A: Yes, our standard service includes basic data analysis (normalization, differential expression analysis, and clustering). Advanced bioinformatics services, including pathway analysis and target prediction, are available as add-on options.

Q: What modifications are available for agomir/antagomir synthesis?

A: We offer various chemical modifications including 2'-O-methyl, 2'-O-MOE, phosphorothioate linkages, and cholesterol conjugation. These modifications can enhance the stability and cellular uptake of the molecules.

Q: What is the minimum purity guaranteed for synthesized agomirs/antagomirs?

A: Our standard synthesis guarantees >90% purity, with options for higher purity levels (>95% or >98%) available upon request. Each batch is accompanied by quality control documentation including HPLC and mass spectrometry data.

Q: Can you design sponges targeting multiple miRNAs simultaneously?

A: Yes, we can design multi-target sponges containing binding sites for different miRNAs. This is particularly useful for studying miRNA families or multiple miRNAs involved in the same pathway. We can help optimize the design to maintain effectiveness while targeting multiple sequences.

Q: What quality control measures are in place for these services?

A: Each service includes comprehensive quality control steps including:

  • RNA quality assessment (RIN score, concentration, purity)
  • Sequence verification for synthetic products
  • Functional validation where applicable
  • Detailed documentation and quality reports
Publications
  1. Wang G, Zhang Z, Xia C. Long non-coding RNA LINC00240 promotes gastric cancer progression via modulating miR-338-5p/METTL3 axis[J]. Bioengineered, 2021, 12(2): 9678-9691.
  2. Zhang Y, Meng W, Yue P, et al. RETRACTED ARTICLE: M2 macrophage-derived extracellular vesicles promote gastric cancer progression via a microRNA-130b-3p/MLL3/GRHL2 signaling cascade[J]. Journal of Experimental & Clinical Cancer Research, 2020, 39(1): 1-20.
* For research use only. Not intended for any clinical use.
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