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| General Information | |
| Organism | Gallus gallus, chicken |
| Cell Line Description | The DT40 chicken B cell line is derived from avian leukemia virus (ALV)-induced bursa lymphoma. ALV was injected intravenously into Hyline SC chickens, and the resulting tumors were transplanted twice in vivo before cell culture. DT40 cells are small, approximately 10 μm in diameter, and have a high nuclear-to-cytoplasmic ratio. They carry IgM on their surface. DT40 cells do release ALV into the surrounding environment, but in negligible amounts. The DT40 cell line is a versatile system that can be used to study specific signal transduction pathways, such as those activated by BCR or induced by apoptotic factors, as well as the study of nucleic acid modification, repair, and tissue. DT40 is easy to culture, and mutations in Ig genes can be easily measured by using FACS to quantify the loss of surface immunoglobulins. Furthermore, gene targeting efficiency in DT40 was very high, possibly due to elevated levels of enzymes involved in homologous recombination for gene conversion. This makes DT40 a useful model for analyzing gene function. |
| Tissue | Bursa |
| Disease | Lymphoma |
| Morphology | Lymphoblast |
| Age | 1 day |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Genetic manipulation and gene targeting 2. DNA repair studies 3. Signal transduction research 4. Drug screening and pharmacology 5. Immunology and infectious disease research 6. Developmental biology research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The use of the chicken DT40 B cell line is becoming increasingly popular due to its ease of genetic manipulation. It provides a targeted DNA to random DNA integration ratio of over 1:2, far exceeding that of any mammalian cell line. The facility to generate knockout cell lines combined with the short generation time makes the DT40 cell line attractive for phenotypic analysis of single- and multigene disruptions. |
| Characteristics | |
| Genes Expressed | Immunoglobulin, IgM (surface) |
| Oncogene | C-myc+ |
| Tumorigenic | Yes, in 2-day-old syngeneic chickens. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Cultures can be maintained by adding or replacing fresh medium. Start culture at 3 x 105 cells/mL and maintain between 2 x 105 and 2 x 106 cells/mL. |
| Medium Renewal | Every 2 to 3 days |
| Culture Medium | Dulbecco's modified Eagle's medium with 4 mM L-glutamine modified to contain 4.5 g/L glucose, 1.5 g/L sodium bicarbonate and 0.05 mM 2-mercaptoethanol, 75%; tryptose phosphate broth, 10%; fetal bovine serum, 10%; chicken serum, 5% |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| RTA-0010 | DT40-Insulin Assay Cells | Inquiry |
| RTA-0012 | DT40-IL2 Assay Cells | Inquiry |
| RTA-0014 | DT40-IL23 Assay Cells | Inquiry |
| RTA-0015 | DT40-IL12 Assay Cells | Inquiry |
| RTA-0016 | DT40-GCSF Assay Cells | Inquiry |
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