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| General Information | |
| Organism | Rattus norvegicus, rat |
| Cell Line Description | PC-12 is a cell line derived from a rat adrenal medullary pheochromocytoma, which is embryonically derived from the neural crest and contains a mixture of neuroblasts and eosinophils. The cell line was first cultured by Greene and Tischler in 1976. Studies of dopamine regulation have shown that PC12 cells synthesize, release, and reuptake dopamine, and the presence of neurosecretory and ion channels and neurotransmitter receptors have been extensively characterized. The popularity of PC-12 cells is primarily due to their extreme flexibility in pharmacological manipulation, ease of culture, and the large amount of background knowledge available on their proliferation and differentiation. PC-12 cells grown under normal conditions have morphological, physiological, and biochemical characteristics of adrenal cells. When cultured in the presence of nerve growth factor (NGF), they differentiate morphologically and functionally into sympathetic ganglion neurons. PC-12 cells synthesize and store dopamine and are a suitable model for studying catecholamine neurotoxicity in vitro. PC-12 cells have been widely used to study the neurotoxic activity of various substances, for example by evaluating the effects on cell survival, neurite outgrowth, DNA damage, or protein expression levels. |
| Tissue | Adrenal gland |
| Disease | Pheochromocytoma |
| Morphology | Polygonal |
| Age | Unspecified |
| Gender | Male |
| Product Format | Frozen |
| Growth Mode | Mixed: floating aggregates of round cells with some attached cells |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Neuroscience Research 2. Neurotoxicity Testing 3. Signal Transduction Studies 4. Drug Screening 5. Neurodegenerative Disease Models 6. Gene Expression Studies 7. Electrophysiological Studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Treatment of PC-12 cells with nerve growth factor produces cells with long processes (called neurite bulges) that contain small numbers of vesicles. After treatment of PC12 cells with nerve growth factor for 10-14 days, no vesicles were released from the cell body, indicating that vesicles accumulate at the neurite terminals. |
| Characteristics | |
| Karyotype | 40 chromosomes; 38 autosomes plus XY |
| Receptors Expressed | Nerve growth factor (NGF) |
| Genes Expressed | Catecholamines: dopamine; norepinephrine |
| Tumorigenic | Yes, in New England Deaconess Hospital strain rats. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The volumes used in this protocol are for 75 cm2 flasks; for culture vessels of other sizes, the amount of dissociation medium should be proportionally reduced or increased. 1. Transfer the cell suspension to a centrifuge tube. Centrifuge the cells at 180 to 225 x g for 8-15 minutes at room temperature. 2. Remove and discard the supernatant, leaving the cell pellet. 3. Resuspend the cell pellet in 5 mls of fresh medium. 4. Gently aspirate each 5 ml of cells 4 or 5 times with a new 20 ml syringe equipped with a 22g needle to break up cell clusters. 5. Add the appropriate cell suspension to a new 75 cm2 flask containing 10-15 ml of fresh growth medium. Inoculate flasks with 5 x 10^5 to 1 x 10^6 viable cells/ml, or use a 1:2 to 1:4 subculture ratio. 6. Place the culture container in a 37℃ incubator and subculture when the cell density reaches 2-4 x 10^6 viable cells/mL. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:3 to 1:8 is recommended. |
| Culture Medium | RPMI 1640 + 2mM Glutamine + 10% Horse Serum + 5% Foetal Bovine Serum (FBS) |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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