Ion Channel Cell Line Development

Introduction

Ion channels are a large family of integral, pore-forming membrane proteins, typically formed by multi-subunits, and exist in the membranes of all cells. Upon stimulating by chemical or mechanical signals, ion channels open or close to allow ions to move into or out of the cell. An ion passing through the channel pore generates electrical signals which can be monitored using electrophysiological methods.

There are several types of ion channels, for example, sodium, potassium and calcium channels, which are involved in a wide variety of biological processes. In addition, dysfunction of ion channels can lead to various diseases. These features make ion channels as important therapeutic targets for treating a number of diseases. Although discovery of drugs targeting ion channels is difficult, with the improvement in methodologies such as cell-based high throughput screening (HTS), there is an opportunity to characterize and identify promising channel drug targets.

Leading Capability

Creative Biogene offers an integrated custom service, starting with gene synthesis through to generation of ion channel stable cell lines and cell-based ion channel assays. Scientists at Creative Biogene are dedicated to advance ion channel discovery. So far, we have successfully developed hundreds of cell lines stably expressing functional ion channels. Our extensive collection of ion channel stable cell lines can be particularly useful in compound screening and early stage drug discovery programs. The proven expertise of our scientific team and the amenable portfolio of developing system, enable us to create the optimized ion channel stable cell lines to fulfill all of the ion channel discovery needs of our clients. By outsourcing the stable cell line development to Creative Biogene, customers can circumvent time-consuming and tedious cell line generation work and focus on generating more challenging scientific ideas.

Target Experience	Target Species	Assay Platform
Sodium channels Potassium channels Calcium channels Chloride channels TRP channels P2X channels Other channels	Human Mouse Rat Monkey Dog Pig	Manual electrophysiology Automated electrophysiology Fluorescence RT-PCR

Representative data of our ion channel platform

Figure 1. Representative data of our ion channel platform

Applications

Study of ion channel functions and mechanisms
Identification of ion channel targeting drug candidates
High thoughput screening
High content screening

Advantages

Our scientists have extensive experience with ion channels.
Our integrated services include gene synthesis, stable cell line development as well as cell-based assays.
We offer flexible host cell options or you can provide your own.
We can qualify cells for assay performance on a variety of platforms.
Our proprietary platforms enable us to generate stable cell lines co-expression of multiple ion channel subunits.

References:

Camerino DC, Tricarico D, Desaphy JF (April 2007). "Ion channel pharmacology". Neurotherapeutics. 4 (2): 184–98.
Verkman AS, Galietta LJ (February 2009). "Chloride channels as drug targets". Nature Reviews. Drug Discovery. 8 (2): 153–71.
Camerino DC, Desaphy JF, Tricarico D, Pierno S, Liantonio A (2008). "Therapeutic approaches to ion channel diseases". Advances in Genetics. 64: 81–145.
Vicini S (April 1999). "New perspectives in the functional role of GABA(A) channel heterogeneity". Molecular Neurobiology.
Jiang Y, Lee A, Chen J, Ruta V, Cadene M, Chait BT, MacKinnon R (May 2003). "X-ray structure of a voltage-dependent K+ channel". Nature. 423 (6935): 33–41.

* For research use only. Not intended for any clinical use.

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