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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | T98G is a highly specialized human glioblastoma model derived from a 61-year-old Caucasian male patient. Established by G.H. Stein in 1979, this cell line, unlike other transformed cell lines, is capable of G1 phase arrest under high-density or serum-deprivation conditions—a characteristic typically lost in cancer cells. These cells exhibit a fibroblast-like morphology and grow in an adherent monolayer. Biologically, T98G is characterized by significant chromosomal instability and a hyperpentaploid karyotype. It is a crucial model for studying proliferation and quiescent transitions, as well as the molecular mechanisms of glioblastoma drug resistance. |
| Tissue | Brain |
| Disease | Glioblastoma Multiforme (GBM) |
| Morphology | Fibroblast-like |
| Gender | Male |
| Age | 61 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 |
| Applications | 1. Glioblastoma Pathogenesis Research 2. Cell Cycle and Checkpoint Studies 3. Neuropharmacology and Drug Testing 4. Virology and Viral Latency Study 5. Targeted Gene Editing (CRISPR/Cas9) |
| Shipped In | Dry ice |
| Storage Temperature | Below −140°C or in liquid nitrogen vapor phase |
| Characteristics | |
| Karyotype | Highly aneuploid; Hyperpentaploid with a modal chromosome number of 128 to 132. |
| Tumorigenic | No (non-tumorigenic in nude mice), demonstrating that anchorage independence in this line does not necessarily correlate with in vivo tumor formation. |
| DNA Profile (STR) | Amelogenin: X; CSF1PO: 10, 11; D13S317: 8, 11; D16S539: 12; D5S818: 11, 12; D7S820: 8, 9; TH01: 9.3; TPOX: 8; vWA: 15, 17. |
| Mutations | Homozygous TP53 mutation; PTEN mutation; harbors hyperactive Jak2 signaling. |
| Markers Expressed | Positive for glial markers including Vimentin, Glutamine Synthase, and Glial Fibrillary Acidic Protein (GFAP). |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with calcium- and magnesium-free PBS buffer or 0.25% trypsin-EDTA solution to remove all serum residue. 3. Add 2.0 to 3.0 mL of 0.25% (w/v) trypsin-0.53 mM EDTA solution (for T75 culture flasks). 4. Observe under an inverted microscope until the cell layer disperses (usually 5 to 15 minutes). For cells that are difficult to disperse, incubate at 37°C. 5. Add an equal volume of complete culture medium to neutralize the trypsin. 6. Gently pipette the cells and transfer them to centrifuge tubes. 7. Centrifuge at 125 x g for 5 to 7 minutes. 8. Resuspend the cell pellet in fresh complete culture medium and aliquot into new culture containers. |
| Medium Renewal | 3 to 4 times per week. |
| Subcultivation Ratio | A split ratio of 1:3 to 1:10 is recommended. |
| Culture Medium | EMEM (Eagle's Minimum Essential Medium) + 10% Fetal Bovine Serum (FBS) + 2 mM L-Glutamine + 1 mM Sodium Pyruvate + 1% NEAA. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | 90% to 95% complete growth medium supplemented with 5% to 10% (v/v) DMSO. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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