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| General Information | |
| Organism | Sus scrofa, Pig |
| Cell Line Description | IPEC-J2 cells are intestinal porcine enterocytes isolated from the jejunum of neonatal, non-sucking piglets. The IPEC-J2 cell line is unique in that it is derived from the small intestine and is neither transforming nor tumorigenic in nature. Given the correct culture conditions, these cells will divide and grow for an infinite number of passages in vitro. IPEC-J2 cells mimic human physiology more closely than any other cell line of non-human origin. IPEC-J2 cells were first used to study transepithelial ion transport and intestinal epithelial cell differentiation. They have proven to be valuable tools for characterizing epithelial cell interactions with intestinal bacteria and viruses, providing insights into initial host-pathogen and non-pathogen (e.g. commensal or probiotic) interactions. |
| Tissue | Intestine |
| Morphology | Epithelial |
| Gender | Sex unspecified |
| Age | A neonatal, unsuckled pig |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | IPEC-J2 cell is an ideal tool to study epithelial transport, interactions with enteric bacteria, effects of probiotic microorganisms and the effect of nutrients and other feedstuffs on a variety of widely used parameters (e.g. transepithelial electrical resistance (TEER), permeability, metabolic activity) reflecting epithelial functionality. |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Compared with transformed cell lines, IPEC-J2 cells have two major advantages as a model of normal intestinal epithelial cells: (1) as permanent cell lines, they maintain their differentiation characteristics and exhibit strong similarity to primary intestinal epithelial cells, (2) The advantage of IPEC-J2 cells is that they can be directly compared with experimental animals used as human in vivo models. Among all non-primates, the porcine gastrointestinal tract is the most suitable in vivo model because it is similar to the human gastrointestinal system in terms of size, weight, anatomy, and physiology. |
| Characteristics | |
| Genes Expressed | Claudin-1, -3, -4, -5, -7, -8, -12, tricellulin, occludin, E-cadherin, zonula occludens-1 (ZO-1), cytokines, defensins, toll-like receptors, mucins and F4 fimbrial receptor |
| Tumorigenic | No |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Rinse the cell layer briefly with DPBS solution to remove all traces of serum containing trypsin inhibitors. 3. Add 1.0 to 2.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 4. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. |
| Medium Renewal | Every 3 to 5 days |
| Subcultivation Ratio | The ratio of 1:2 to 1:5 is recommended. |
| Culture Medium | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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