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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | BxPC-3 cells, derived from a pancreatic adenocarcinoma in a 61-year-old female patient who underwent radiation and chemotherapy, have become a fundamental asset in cancer research, particularly in the study of pancreatic ductal adenocarcinoma. The lack of SMAD4/DPC4 protein due to homozygous deletion in BxPC 3 cells makes them a valuable resource for studying the genetic landscape of pancreatic cancer. The BXPC-3 cell line has several properties that make it a useful tool in cancer research. It was able to grow in culture and exhibited many of the same properties as pancreatic cancer cells found in patients. Researchers used the BXPC-3 cell line to study the molecular mechanisms of pancreatic cancer, including genes and pathways involved in cancer cell growth, invasion and metastasis. The BXPC-3 cell line has also been used to test the efficacy of various cancer treatments. It will be particularly useful in developing drugs that target the epidermal growth factor receptor (EGFR) and mitogen-activated protein kinase (MAPK) signaling pathways, which are frequently dysregulated in pancreatic cancer. |
| Tissue | Pancreas |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 61 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Studying pancreatic cancer biology 2. Drug screening and discovery 3. Target identification 4. Biomarker discovery |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | BxPC-3 cells' significant expression of angiogenic factors such as interleukin-8 (IL-8), vascular endothelial growth factor (VEGF), and prostaglandin E2 (PGE2) opens avenues for exploring angiogenesis in cancer progression and identifying potential therapeutic targets. |
| Characteristics | |
| Genes Expressed | mucin; pancreas cancer specific antigen (pancreas cancer associated antigen); carcinoembryonic antigen |
| Tumorigenic | Yes |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37°C. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:3 to 1:6 is recommended. |
| Culture Medium | RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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