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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The A375 cell line was derived from skin cells of a 54-year-old female patient with malignant melanoma. A375 is a suitable host for skin cancer research as well as human skin infections. The A375 cell line is known for its rapid growth rate and high tumorigenic potential, making it suitable for a variety of experimental applications, including in vitro studies of cell proliferation, migration, and invasion, as well as in vivo tumorigenesis assays. A375 cells exhibit high tumorigenic potential in immunosuppressed mice, forming rapidly growing melanoma-free tumors. In addition to their role in basic research on melanoma, A375 cells are also used in drug screening and research on signaling pathways related to cancer cell survival, proliferation, and metastasis. A375 cells have been further used for apoptosis research, and the introduction of genetic cell lines such as A375 and reporter proteins such as luciferase has enabled the study of gene function and real-time monitoring of cellular responses. The suitability of A375 cells as transfection hosts and their use in stable reporter cell lines also contribute to their versatility in research applications. |
| Tissue | Skin |
| Disease | Malignant Melanoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 54 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Studying melanoma biology 2. Drug screening 3. Gene expression studies 4. Signaling pathway analysis 5. High-throughput screening 6. Toxicology studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The presence of the BRAFV600E mutation in A375 cells renders them highly sensitive to MEK inhibition, providing a valuable tool for studying targeted therapies in melanoma treatment. For example, treatment of A375 cells with vemurafenib has been shown to enhance the induction of MHC class I and II molecules, providing insights into the interaction between melanoma cells and the immune system. |
| Characteristics | |
| Antigen Expression | P53 positive |
| Karyotype | It is a subtriploidy with a chromosome mode of 62. There are 9 marker chromosomes commonly found in each cell, with normal N2, N6, and N22 present in one copy per cell. |
| Tumorigenic | Yes, in immunosuppressed mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37°C. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:3 to 1:8 is recommended. |
| Culture Medium | DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | 95% of complete medium + 5% v/v DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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