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K562 Cell Line


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K562 Cell Line

General Information
OrganismHomo sapiens, human
Cell Line DescriptionK562 cells were established the first human immortalized myelogenous leukemia line. This cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. They can be used as a highly sensitive target for the in vitro natural killer assay. Recently, studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the granulocyte, erythrocyte and monocytic series.
TissueBone marrow
MorphologyLymphoblast
DiseaseChronic myelogenous leukemia (CML)
Age53 years
PrimaryNo
GenderFemale
Product FormatFrozen
Growth ModeSuspension
Biosafety Level1
Biosafety classification is based on U.S. Public Health Service Guidelines. It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ApplicationsCell culture/growth conditions, stable cell transfection, transient transfection, gene expression, protein expression, protein purification
Shipped inDry ice
Storage Temperature−196°C
Characteristics
Karyotype2n = 46
ImagesK562 Cell Line
Antigen ExpressionCD7 (25%)
Cytogenic DataThe stemline chromosome number is triploid.
MycoplasmaContamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays
ImmunologyCD3 -, CD14 -, CD15 +, CD19 -, CD33 +, CD71 +, CD235a +
VirusesELISA: reverse transcriptase negative; PCR: HBV -, HCV -, EBV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
TumorigenicYes
EffectsYes, in nude mice
Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Culture Conditions and Handling
Culture MediumRPMI 1640 + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).
MorphologyRound large, single cells in suspension
SubculturingCultures can be maintained by the addition or replacement of fresh medium. Seed out at ca. 0.5 x 106 cells/ml, and maintain at 0.1-1.0 x 106 cells/ml. Viability may be low after thawing, but cells recover during the following 2-3 days. Starting the culture with 20% FBS in 12- or 24-well-plates could be of advantage.
Split RatioSplit 1:3 to 1:5
HarvestMaximal density at 1.0-1.5 x 106 cells/ml
Medium RenewalAdd fresh medium every 2 to 3 days
Freeze MediumComplete culture medium supplemented with 5% (v/v) DMSO
Culture Temperature37°C
Incubation ConditionCarbon dioxide (CO2), 5%

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