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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | A20 is a murine B-lymphoma cell line derived from BALB/c mice with spontaneous reticular cell tumors. It is often used as a model to study B-cell lymphomas and their response to various treatments. The A20 cell line has several properties that make it a useful tool for cancer research. It is able to grow in culture and is tumorigenic when injected into mice. It is also highly sensitive to apoptosis, so it can be used to study the mechanisms of cell death and survival of cancer cells. Researchers have used the A20 cell line to study the molecular mechanisms of B-cell lymphoma, including genes and pathways involved in cancer cell growth and survival. The A20 cell line has also been used to test the efficacy of various cancer treatments, including chemotherapy, radiation therapy, and immunotherapy. |
| Disease | Sarcoma; Reticulum Cell |
| Morphology | Lymphoblast |
| Gender | Unspecified |
| Age | >15 months |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Immunology research 3. 3D cell culture 4. Drug screening and discovery |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | A20 cells can present alloantigens and protein antigens. A20 cells were tested and found to be negative for ectromelia virus (mousepox). |
| Characteristics | |
| Karyotype | Diploid; modal number = 37; range = 33 to 38 |
| Tumorigenic | Yes |
| Genes Expressed | Immunoglobulin (surface, sIg+) I-A+ |
| Expression Markers | Fc |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Suspended cells: Remove cells from the matrix with fresh medium. To obtain single cells, pass the suspension several times with a 22-gauge needle and aliquot into new flasks. Growing on collagen: To remove adherent cells, use the following standard protocol. Remove medium and rinse adherent cells with PBS without calcium and magnesium (3-5 ml PBS for T25 flasks and 5-10 ml for T75 flasks). Add TrypleExpress (1-2 ml per T25 flask and 2.5 ml per T75 flask), the cell sheet must be completely covered. Incubate at 37℃ for 10 minutes. Carefully resuspend cells, adding medium is optional but not necessary, and aliquot into new flasks containing fresh medium. |
| Medium Renewal | Every 2 to 3 days. |
| Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Culture Conditionsc | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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