Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Cricetulus griseus, Chinese hamster |
| Cell Line Description | V79 is a widely utilized fibroblast-like cell line, originally isolated in 1958 from the lung tissue of a male Chinese hamster. In the fields of genetic toxicology and radiation biology, it stands as one of the most significant mammalian cell models. V79 cells are characterized by their exceptionally rapid growth rate (with a doubling time of 10–14 hours), high cloning efficiency, and relatively simple karyotype. These cells are frequently employed to investigate gene mutations, chromosomal aberrations, and DNA repair mechanisms. Given their lack of significant endogenous metabolic activation enzymes (such as cytochrome P450), this cell line is often used in conjunction with an exogenous metabolic system (S9 mix) to assess the mutagenicity of pro-carcinogens. |
| Tissue | Lung |
| Cell Type | Fibroblast |
| Morphology | Fibroblast-like |
| Gender | Male |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Genetic toxicology and mutagenicity assays (e.g., HPRT gene mutation assay) 2. Radiation biology and DNA damage/repair research 3. Screening for environmental pollutants and industrial chemicals 4. Cytotoxicity testing and evaluation of drug safety 5. Study of cell cycle kinetics and centrosome functions |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Near-diploid; modal number = 22 (range 19–23) |
| Growth Kinetics | Extremely rapid; doubling time approximately 12 hours |
| Genetic Profile | TP53 deficient (missense mutation); stable chromosomal structure |
| Cloning Efficiency | Very high (>80%); ideal for single-cell colony formation assays |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe until the cell monolayer has dispersed (typically takes 2 to 5 minutes). 4. Add complete growth medium to neutralize the trypsin, and gently pipette to prepare a single-cell suspension. 5. Transfer the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:5 to 1:10 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02573 | Cas9 Stable Cell Line - V79 | Inquiry |
| CSC-RR00888 | GFP Reporter Cell Line - V79 | Inquiry |
| CSC-RR00937 | Luciferase Reporter Cell Line - V79 | Inquiry |
Copyright © Creative Biogene. All rights reserved.