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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | ZR-75-1 is a human breast cancer cell line commonly used in research. Derived from a malignant ascitic effusion in a 63 year old female Caucasian with infiltrating ductal carcinoma. The ZR-75-1 cell line is known to be estrogen receptor positive, making it a useful model for studying hormone-dependent breast cancer. It is commonly used to study the molecular mechanisms of breast cancer progression and to test the efficacy of potential anticancer drugs. |
| Tissue | Breast |
| Disease | Carcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 63 years |
| Product Format | Frozen |
| Growth Mode | Adherent or attaching to the culture flask |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | ZR-75-1 cell lines are powerful tools for studying breast tumor progression, molecular and cellular mechanisms of treatment resistance, and for testing the effectiveness of new drugs against different patterns of antiestrogen insensitivity. |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | ZR-75-1 cells were infected with amphiphilic, defective murine retrovirus and plated in medium containing 1uM 4-hydroxy-tamoxifen. Within 5 weeks of initiation of selection, proliferating colonies were picked individually and expanded into stable cell lines. |
| Characteristics | |
| Karyotype | This human cell line has a hypertriploid chromosome number. The chromosome mode in 26% of the cells was 72, and the proportion of higher ploidy was 1.2%. Eighteen marks are common. |
| Tumorigenic | Yes, tumors form in nude mice. |
| Mycoplasma Test | Negative |
| Cytogenetic Information | These cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA, but do not express the MUC-3 gene. |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:4 to 1:6 is recommended. |
| Culture Medium | RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (RBCS) |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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