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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | MIN6 (mouse insulinoma 6) is a pancreatic β-cell line derived from transgenic mice expressing the SV40 T antigen, which is expressed under the control of the insulin promoter. MIN6 is one of the most widely used models in diabetes research because it preserves key physiological characteristics of pancreatic β-cells, particularly glucose-stimulated insulin secretion (GSIS). MIN6 cells exhibit a dose-dependent insulin response to glucose concentrations within physiological ranges, similar to normal islets. They are crucial for studying insulin biosynthesis, secretion mechanisms, and the protective or detrimental effects of various compounds on β-cell function. |
| Tissue | Pancreas |
| Disease | Insulinoma |
| Morphology | Epithelial-like; cells typically grow in clusters or small aggregates |
| Gender | Not specified (derived from IT6 transgenic mice) |
| Age | Adult (approx. 13-18 weeks at isolation) |
| Product Format | Frozen |
| Growth Mode | Adherent (often forming clusters) |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Study of glucose-stimulated insulin secretion (GSIS) mechanisms 2. Diabetes and metabolic disease research 3. Drug screening for insulinotropic agents 4. Beta-cell signaling and gene expression studies 5. Investigation of beta-cell apoptosis and survival pathways |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, forms insulin-producing tumors in syngeneic mice |
| Expression Markers | Positive for Insulin, Glucokinase (GK), and GLUT2 |
| Specific Function | Retains glucose-responsive insulin secretion (typically between 5 mM and 25 mM glucose) |
| Passage Sensitivity | High passage numbers (typically >40) may lead to a loss of glucose-stimulated insulin response |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca²⁺/Mg²⁺-free PBS buffer to remove residual serum. 3. Add trypsin-EDTA solution (0.05% or 0.25%) and incubate at 37°C for 3 to 5 minutes, until cells begin to detach. 4. Add complete culture medium to neutralize the trypsin and gently pipette to disperse cell clumps. 5. Seed the cells into new culture dishes at the recommended ratio. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Medium | DMEM (High Glucose) supplemented with 10% to 15% Fetal Bovine Serum (FBS) and 50 µM beta-mercaptoethanol (essential for maintaining the insulin-secreting phenotype). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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